Pdx1-RFP puro (DM#235)
Full plasmid sequence is not available for this item.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||15085||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Vector backbonepBluescript KS II
- Backbone size w/o insert (bp) 3000
Vector typeBacterial Expression
Growth in Bacteria
Bacterial Resistance(s)Ampicillin, 100 μg/mL
Copy numberHigh Copy
Gene/Insert namePdx1 promoter
SpeciesM. musculus (mouse)
Entrez GenePdx1 (a.k.a. IDX-1, IPF-1, Ipf1, Mody4, STF-1, pdx-1)
/ Fusion Protein
- RFP (C terminal on insert)
- Cloning method Restriction Enzyme
- 5′ cloning site XbaI (not destroyed)
- 5′ sequencing primer T3
- 3′ sequencing primer T7 (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
This plasmid is designed to sort out Pdx1 expressing cells by puromycin resistancy. See author's map for more information.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:Pdx1-RFP puro (DM#235) was a gift from Douglas Melton (Addgene plasmid # 15085 ; http://n2t.net/addgene:15085 ; RRID:Addgene_15085)
For your References section:Insulin staining of ES cell progeny from insulin uptake. Rajagopal J, Anderson WJ, Kume S, Martinez OI, Melton DA. Science. 2003 Jan 17. 299(5605):363. 10.1126/science.1077838 PubMed 12532008
Map uploaded by the depositor.