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PE8a
(Plasmid #257698)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 257698 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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Backbone

  • Vector backbone
    pT7
  • Modifications to backbone
    Inactivated T7 promoter to prevent transcription from plasmid
  • Vector type
    Template for in vitro transcription

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    PE8a prime editor
  • Species
    Synthetic
  • Promoter T7 (inactivated)

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    PE8a was a gift from David Liu (Addgene plasmid # 257698 ; http://n2t.net/addgene:257698 ; RRID:Addgene_257698)

  • For your References section:

    AI-guided redesign of laboratory-evolved reverse transcriptases enhances prime editing. Tao YA, Sakai HA, Jiang AY, Krasnow NA, Vaganov VS, Shim B, Barsdale Z, Pandey S, Ahmed N, Na M, Liao TW, Oye K, Cristian A, Zhang E, Xu JA, Bulcaen M, Liu DR. Nat Biotechnol. 2026 May 21. doi: 10.1038/s41587-026-03149-6. 10.1038/s41587-026-03149-6 PubMed 42168701
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