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A high-throughput assay for phosphoprotein-specific phosphatase activity in cellular extracts.
Bose AK, Janes KA
Mol Cell Proteomics. 2013 Mar;12(3):797-806. doi: 10.1074/mcp.O112.024059. Epub 2012 Dec 11.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
47573pGEX-4T-1 3xFlag-ERK2Bacterial expression plasmid for GST-3xFlag-tagged ERK2
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47574pGEX-4T-1 3xFlag-JNK1a1Bacterial expression plasmid for GST-3xFlag-tagged JNK1a1
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47575pGEX-4T-1 3xFlag-p38alphaBacterial expression plasmid for GST-3xFlag-tagged p38alpha
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47576pGEX-4T-1 3xHA-MEK1-DDBacterial expression plasmid for GST-3xHA-tagged constitutively active MEK1
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47577pGEX-4T-1 3xHA-MKK4-EEBacterial expression plasmid for GST-3xHA-tagged constitutively active MKK4
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47578pGEX-4T-1 3xHA-MKK7a1-EEBacterial expression plasmid for GST-3xHA-tagged constitutively active MKK7a1
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47579pGEX-4T-1 3xHA-MKK6-EEBacterial expression plasmid for GST-3xHA-tagged constitutively active MKK6
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47580pCDFDuet-1 MKK4(EE)-MKK7a1(EE)Bacterial expression plasmid for co-expression of constitutively active MKK4 and MKK7a1
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