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pCDFDuet-1 MKK4(EE)-MKK7a1(EE)
(Plasmid #47580)

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Full plasmid sequence is not available for this item.

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Item Catalog # Description Quantity Price (USD)
Plasmid 47580 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pCDFDuet-1
  • Backbone manufacturer
    Novagen
  • Backbone size w/o insert (bp) 3781
  • Total vector size (bp) 5851
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Spectinomycin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    BL21
  • Copy number
    Low Copy

Gene/Insert 1

  • Gene/Insert name
    MKK4-EE
  • Alt name
    MAP2K4
  • Alt name
    MEK4
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1092
  • Mutation
    Lacks the first 36 residues of the RefSeq protein. Ser257 to Glu, Thr261 to Glu
  • GenBank ID
    NM_003010
  • Entrez Gene
    MAP2K4 (a.k.a. JNKK, JNKK1, MAPKK4, MEK4, MKK4, PRKMK4, SAPKK-1, SAPKK1, SEK1, SERK1, SKK1)

Cloning Information for Gene/Insert 1

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site SalI (not destroyed)
  • 5′ sequencing primer ACYCDuetUP1
  • 3′ sequencing primer DuetDOWN1
  • (Common Sequencing Primers)

Gene/Insert 2

  • Gene/Insert name
    MKK7a1-EE
  • Alt name
    Map2k7
  • Alt name
    Mek7
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    1041
  • Mutation
    Residues 73-420 of RefSeq sequence. Ser271 to Glu, Thr275 to Glu.
  • GenBank ID
    NM_001042557

Cloning Information for Gene/Insert 2

  • Cloning method Restriction Enzyme
  • 5′ cloning site BglII (destroyed during cloning)
  • 3′ cloning site XhoI (destroyed during cloning)
  • 5′ sequencing primer DuetUP2 Primer
  • 3′ sequencing primer T7 Terminator Primer
  • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Addgene plasmids #47577 and #47578.
  • Terms and Licenses
  • Industry Terms
    • Not Available to Industry
  • Article Citing this Plasmid
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCDFDuet-1 MKK4(EE)-MKK7a1(EE) was a gift from Kevin Janes (Addgene plasmid # 47580 ; http://n2t.net/addgene:47580 ; RRID:Addgene_47580)
  • For your References section:

    A high-throughput assay for phosphoprotein-specific phosphatase activity in cellular extracts. Bose AK, Janes KA. Mol Cell Proteomics. 2013 Mar;12(3):797-806. doi: 10.1074/mcp.O112.024059. Epub 2012 Dec 11. 10.1074/mcp.O112.024059 PubMed 23233447