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pRS327
(Plasmid #51787)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 51787 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pRS423
  • Modifications to backbone
    The CEN plasmid pRS327 was constructed by homology mediated recombination in yeast. A 1.84-kb ApaLI fragment from pRS423 that has the 2-μm origin of replication was transformed into lys2 yeast along with plasmid pRS307 that had been linearized with AatII. Selection for Lys+ prototrophs identified yeast where recombination of the homologous regions between the two plasmids created a circular DNA molecule with the 2-μm origin of DNA replication and the LYS2 marker.
  • Vector type
    Yeast Expression
  • Selectable markers
    LYS2

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Cloning Information

Resource Information

  • Terms and Licenses
  • Industry Terms
    • Not Available to Industry
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pRS327 was a gift from David Stillman (Addgene plasmid # 51787 ; http://n2t.net/addgene:51787 ; RRID:Addgene_51787)
  • For your References section:

    pRS yeast vectors with a LYS2 marker. Eriksson P, Thomas LR, Thorburn A, Stillman DJ. Biotechniques. 2004 Feb;36(2):212-3. 10.2144/04362BM01 PubMed 14989082