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pBEVY-GA
(Plasmid #51227)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 51227 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pBEVY-GT
  • Backbone manufacturer
    Addgene plasmid 51231
  • Modifications to backbone
    pBEVY-GA was made by cutting pBEVY-GT with AatII and XcmI to remove the TRP1 gene. A fragment containing the ADE2 gene derived from a BglII digest of the pASZ11 plasmid was inserted using blunt ligation to generate pBEVY-GA.
  • Vector type
    Yeast Expression
  • Promoter GAL1,10
  • Selectable markers
    ADE2

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Cloning Information

Resource Information

  • Terms and Licenses

Depositor Comments

Please note that there are minor discrepancies between Addgene's quality control sequence and depositor's reference sequence. The changes are in cloning junctions and should not affect plasmid function.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pBEVY-GA was a gift from Charles Miller (Addgene plasmid # 51227 ; http://n2t.net/addgene:51227 ; RRID:Addgene_51227)
  • For your References section:

    Assessment of aryl hydrocarbon receptor complex interactions using pBEVY plasmids: expressionvectors with bi-directional promoters for use in Saccharomyces cerevisiae. Miller CA 3rd, Martinat MA, Hyman LE. Nucleic Acids Res. 1998 Aug 1;26(15):3577-83. 10.1093/nar/26.15.3577 PubMed 9671822