pBEVY-GL
(Plasmid #51225)

• Purpose: (Empty Backbone) bi-directional expression vector for yeast, galactose inducible, LEU2 selection
• Depositing Lab: Charles Miller
• Publication: Miller CA et al Nucleic Acids Res. 1998 Aug 1;26(15):3577-83.

Backbone

• Vector backbone: YEplac181
• Backbone manufacturer: R.D.Gietz (University of Manitoba)
• Modifications to backbone: To construct the pBEVY-G series of plasmids, the GAL1,10 promoter was amplified by PCR and inserted into the dephosphorylated SmaI site within the polylinker of YEplac181. The GAL1,10 promoter was inserted into the center of the polylinker in an orientation that regenerated a SmaI site 3′ of the GAL1 side of the promoter. EcoRI and HpaI were used to remove a portion of the parent plasmid sequence and an ADH2 terminator having an EcoRI overhang and a blunt end was inserted to provide a termination function for transcription from the GAL1 side of the bi-directional promoter. The ADH1 terminator was digested with HindIII and was inserted into the dephosphorylated HindIII site of the plasmid, placing a second terminator 3′ of the GAL10 gene. The resulting plasmid was called pBEVY-GL because of its galactose-induction and the LEU2 selection gene.
• Vector type: Yeast Expression
• Promoter: GAL1,10
• Selectable markers: LEU2

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ sequencing primer: Gal1 and Gal10Pro_F

Resource Information

• Articles Citing this Plasmid:
  • 3 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please note that there are minor discrepancies between Addgene's quality control sequence and depositor's reference sequence. The changes are in cloning junctions and should not affect plasmid function.