Purpose(Empty Backbone) bi-directional expression vector for yeast, galactose inducible, URA3 selection
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||51229||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Backbone manufacturerR.D.Gietz (University of Manitoba)
Modifications to backboneSimilar to pBEVY-GL, Addgene plasmid 51225
Vector typeYeast Expression
- Promoter GAL1,10
Growth in Bacteria
Bacterial Resistance(s)Ampicillin, 100 μg/mL
Copy numberHigh Copy
- Cloning method Restriction Enzyme
- 5′ sequencing primer Gal1 and Gal10Pro_F (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
Please note that there are minor discrepancies between Addgene's quality control sequence and depositor's reference sequence. The changes are in cloning junctions and should not affect plasmid function.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pBEVY-GU was a gift from Charles Miller (Addgene plasmid # 51229 ; http://n2t.net/addgene:51229 ; RRID:Addgene_51229)
For your References section:Assessment of aryl hydrocarbon receptor complex interactions using pBEVY plasmids: expressionvectors with bi-directional promoters for use in Saccharomyces cerevisiae. Miller CA 3rd, Martinat MA, Hyman LE. Nucleic Acids Res. 1998 Aug 1;26(15):3577-83. 10.1093/nar/26.15.3577 PubMed 9671822