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DNA Replicons for Plant Genome Engineering.
Baltes NJ, Gil-Humanes J, Cermak T, Atkins PA, Voytas DF
Plant Cell. 2014 Jan 17.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
51491pREPpMDC32 with Rep/RepA coding sequence from the mild bean yellow dwarf virus
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51492pREPK219HpREP with a mutation in the ATPase domain (K219H). This is predicted to result in loss of geminivirus replication.
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51493pLSLT-DNA destination plasmid. Contains the bean yellow dwarf virus cis-acting elements (LIR and SIR) in an LIR-SIR-LIR orientation. Contains attR1 and attR2 sites between the LIR and SIR sequence.
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51494pLSLGFPpLSL with GFP followed by the us:NPTII repair template, Rep is not included on this plasmid
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51495pLSLZpLSL with Zif268:FokI coding sequence followed by approximately 3kb 'filler' sequence, Rep is not included on this plasmid
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51496pLSLZ.DpLSL with Zif268:FokI coding sequence followed by us:NPTII repair template , Rep is not included on this plasmid
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51498pLSLTpLSL with T30 TALEN followed by us:NPTII repair template, Rep is not included on this plasmid
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51500pLSLRSingle component plant T-DNA plasmid. Contains cis- and trans-acting replicational elements from the bean yellow dwarf virus in an LIR-SIR-Rep/RepA-LIR orientation.
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51501pLSLGFP.RSingle component LSL vector with GFP. Rep is expressed from the complementary sense LIR promoter. GFP is between LIR and SIR and is driven from a 2X35S promoter. Rep is within the replicon
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51510pLSLZ.D.RSingle component LSL vector. Contains 2x35S:Zif268:FokI followed by the us:NPTII template. This sequence is between the LIR and SIR.
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51511pZLSLD.RSingle component LSL vector. Contains 2x35S:Zif268:FokI outside of the replicon borders. Within the replicon is the us:NPTII template.
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51512p35SZ.DpMDC32 with Zif268:FokI followed by us:NPTII repair template sequence
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51513p35SZpMDC32 with Zif268:FokI followed by approximately 3kb of filler sequence
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51514p35STpMDC32 with T30 TALEN pair followed by the us:NPTII donor molecule
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51517pLSLGUSpLSL with GUS coding sequence followed by 3 kb of filler sequence Rep is not included on this plasmid
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51518pNJB80Gateway entry vector with attL5 and attL2 sites flanking a CmR and ccdB gene. In our hands, the multisite reaction still proceeds efficiently.
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51519pNJB91Gateway entry vector with attL1 and attR5 sites. Between the att sites are two FokI coding sequences transcriptionally linked by a T2A sequence. There is a nos-T after the last FokI.
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51520pNJB98Gateway entry vector with attL5 and attL2 sites flanking us:NPTII repair template sequence
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51521pNJB99Gateway entry vector with attL1 and attR5 sites flanking Zif268:FokI coding sequence
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Antibodies from Article