159,157 results

1. UbC-blasticidine-P2A-TETon-3G transactivator

   Plasmid

   #200191

   Purpose

   A TETon activator, which combined with the DiLiCre 2.0 plasmid, drives the expression of DiLiCre 2.0 upon doxycycline. DiLiCre 2.0 is a cre recombinase that is activated by violet light.

   Depositor

   Jacco van Rheenen

   Article

   Vizoso et al Nat Commun. 2022 Oct 28;13(1):6442. doi:

   Insert

   blasticidine - P2A - TETon-3G transactivator
   

   Use

   Lentiviral

   Tags

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   UbC

   Available since

   Aug. 30, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pALPS env

   Plasmid

   #101325

   Purpose

   Encodes codon optimized JR-CSF env

   Depositor

   Jeremy Luban

   Article

   McCauley et al Nat Commun. 2018 Dec 13;9(1):5305. doi:

   Insert

   JR-CSF env
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   March 14, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. LeGO-Cer2

   Plasmid

   #27338

   Depositor

   Boris Fehse

   Article

   Weber et al Mol Ther. 2008 Apr . 16(4):698-706.

   Insert

   SFFV promoter, Cerulean
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 24, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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4. PGK-SMASH-CTERM-GFP

   Plasmid

   #185776

   Purpose

   Degron-GFP fusion plasmid with restriction sites BamH1 and EcoR1 around GFP for subcloning

   Depositor

   William Sellers, Alessandra Ianari

   Article

   Bondeson et al Nat Commun. 2022 Sep 20;13(1):5495. doi:

   Insert

   GFP
   

   Use

   Lentiviral

   Tags

   V5, SMASh

   Expression

   Mutation

   none

   Promoter

   PGK

   Available since

   Sept. 21, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. TREM2_Halo_N_allele

   Plasmid

   #178122

   Purpose

   Donor vector for endogenous tagging of human TREM2 at the N-terminus with halotag

   Depositor

   iPSC Neurodegenerative Disease Initiative (iNDI), Michael Ward

   Insert

   Halotag (TREM2 Human)
   

   Use

   Donor vector

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Dec. 2, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. R726-M01-892: His6-Halotag-tev-Hs.RAF1(52-131)

   Plasmid

   #159696

   Purpose

   E. coli protein expression of His6-Halotag-tev-Hs.RAF1(52-131)

   Depositor

   Dominic Esposito

   Article

   Lakshman et al J Biol Chem. 2019 Feb 8;294(6):2193-2207

   Insert

   His6-Halotag-tev-Hs.RAF1(52-131) (RAF1 Human)
   

   Use

   Tags

   His6-Halotag-tev

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Oct. 5, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. R989-X08-566: His6-MBP-tev-G-Hs.KRAS4b(1-169) G12C

   Plasmid

   #159540

   Purpose

   E. coli protein expression of His6-MBP-tev-G-Hs.KRAS4b(1-169) G12C

   Depositor

   Dominic Esposito

   Article

   Gillette et al Sci Rep. 2015 Nov 2;5:15916. doi: 10.103

   Insert

   His6-MBP-tev-G-Hs.KRAS4b(1-169) G12C (KRAS Human)
   

   Use

   Tags

   His6-MBP-tev

   Expression

   Bacterial

   Mutation

   G12C

   Promoter

   Available since

   Sept. 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA3-ERK-SPARK

   Plasmid

   #106921

   Purpose

   Express ERK-SPARK, an activity reporter of ERK in mammalian cells.

   Depositor

   Xiaokun Shu

   Article

   Zhang et al Mol Cell. 2018 Jan 3. pii: S1097-2765(17

   Insert

   ERK-SPARK
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 13, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. NG-ABE8-dimer

   Plasmid

   #138497

   Purpose

   A-to-G base editor

   Depositor

   David Liu

   Article

   Richter et al Nat Biotechnol. 2020 Jul;38(7):883-891.

   Insert

   ecTadA(WT)-ecTadA(8e)-nSpCas9 (NG)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 16, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA3-Clover

    Plasmid

    #40259

    Purpose

    GFP with robust performance in protein fusions and useful as FRET acceptor for mRuby2 in a FRET pair that offers bright fluorescence, dynamic range, and photostability while limiting emissions overlap

    Depositor

    Michael Lin

    Article

    Lam et al Nat Methods. 2012 Sep 9. doi: 10.1038/nm

    Has Service

    Cloning Grade DNA

    Insert

    Clover GFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV IE

    Available since

    Sept. 24, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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11. NW9

    Plasmid

    #133442

    Purpose

    Covalently circularized nanodisc NW9 for studying membrane proteins and viral entry.

    Depositor

    Gerhard Wagner

    Article

    Nasr et al Nat Methods. 2017 Jan;14(1):49-52. doi:

    Insert

    NW9
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Jan. 22, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. PB-EF1-6xflag-RT3-3xHaloTag(MtoL)-H2B

    Plasmid

    #106309

    Purpose

    The plasmid utilizes RT3 sequence to minimize the expression of 3xHaloTag (Methionine mutated to Leucine)-H2B for long-term H2B single-molecule imaging.

    Depositor

    James Zhe Liu

    Article

    Liu et al Proc Natl Acad Sci U S A. 2017 Dec 28. p

    Insert

    6xflag-RT3-3xHaloTag(MtoL)-H2B (H2BC3 Human)
    

    Use

    Tags

    6xflag-3xHaloTag(MtoL)

    Expression

    Mammalian

    Mutation

    Promoter

    EF1

    Available since

    March 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pGFP-EB1

    Plasmid

    #17234

    Depositor

    Lynne Cassimeris

    Article

    Piehl et al Mol Biol Cell. 2003 Mar . 14(3):916-25.

    Insert

    EB1 (MAPRE1 Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 11, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAIDFA-EF1a-NmScarlet-mAID

    Plasmid

    #140615

    Purpose

    Expression of OsTIR1F74A and mScarlet-mAID protein in mammalian cells under the control of EF1a promoter

    Depositor

    Tatsuo Fukagawa

    Article

    Nishimura et al Nucleic Acids Res. 2020 Oct 9;48(18):e10

    Insert

    OsTIR1 mutated F74A
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pnsgRNA

    Plasmid

    #172717

    Purpose

    Delivery of guide RNA for prime editing

    Depositor

    Tilmann Weber

    Article

    Tong et al Nat Commun. 2021 Sep 1;12(1):5206. doi:

    Insert

    J23119-gRNA
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. EGFR (del3) L747-E749del, A750P

    Plasmid

    #11015

    Purpose

    Retroviral construct for expressing human EGFR with L747-E749 deletion and A750P mutation in human cells

    Depositor

    Matthew Meyerson

    Article

    Greulich et al PLoS Med. 2005 Oct 4. 2(11):e313.

    Insert

    EGFR (del3) (EGFR Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    L747-E749 deleted, A750P

    Promoter

    Available since

    Dec. 6, 2005

    Availability

    Academic Institutions and Nonprofits only

17. pSHS207 - Bacterial expression plasmid for WT SpCas9

    Plasmid

    #101199

    Purpose

    Bacterial expression plasmid for WT SpCas9

    Depositor

    Keith Joung, Jennifer Doudna

    Article

    Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature

    Insert

    SpCas9
    

    Use

    Tags

    10x His, MBP, and TEV site

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Nov. 6, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. Copia Renilla Control

    Plasmid

    #38093

    Depositor

    Philip Beachy

    Article

    Lum et al Science. 2003 Mar 28;299(5615):2039-45.

    Insert

    renilla luciferase
    

    Use

    Luciferase

    Tags

    Expression

    Insect

    Mutation

    Promoter

    copia

    Available since

    Nov. 12, 2012

    Availability

    Academic Institutions and Nonprofits only

19. pENTR_L1-Turbo-YFP-NLS-STOP-L2

    Plasmid

    #127361

    Purpose

    gateway entry vector for expressing nuclear TurboID-YFP under a promoter of choice

    Depositor

    Dominique Bergmann

    Article

    Mair et al Elife. 2019 Sep 19;8. pii: 47864. doi: 1

    Insert

    TurboID (BirA mutant)
    

    Use

    Gateway entry vector

    Tags

    GS linker, NLS, V5, and YFP

    Expression

    Mutation

    Q65P, I87V, R118S, E140K, Q141R, S150G, L151P, V1…

    Promoter

    no promoter

    Available since

    Sept. 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pPGK-T7/2-CD44v3 (fl)

    Plasmid

    #137814

    Purpose

    expression of CD44 proteins in mammalian cells

    Depositor

    Ursula Gunthert

    Article

    Mielgo et al Cell Death Differ. 2006 Mar;13(3):465-77

    Insert

    CD44v3 (fl) (CD44 Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    full length and N280D in the V3 region

    Promoter

    PGK

    Available since

    Aug. 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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