166,137 results

1. pUC19-T7-3WJdB-T

   Plasmid

   #87308

   Purpose

   Expresses the fluorescence-activating Three-way Junction dimeric Broccoli (3WJdB) aptamer under the control of the T7 promoter.

   Depositor

   Donald Burke

   Article

   Alam et al ACS Synth Biol. 2017 May 26. doi: 10.102

   Insert

   Three-way junction dimeric Broccoli aptamer
   

   Use

   Synthetic Biology

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   May 23, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pGEMS-II

   Plasmid

   #204765

   Purpose

   GEMS sensor system (pCMV-EGFP-m6Areporter-DHFR, Ef1a-APO1-YTH, hPGK-DsRed) (pFM577)

   Depositor

   Kate Meyer

   Article

   Marayati et al Nat Biotechnol. 2024 Jan 2. doi: 10.1038

   Insert

   EGFP-m6Alinker-DHFR
   

   Mutation

   mutated all RACs in EGFP to RGC/RCC

   Promoter

   CMV

   Available Since

   Jan. 2, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLG004 (RT-Abi-P2)

   Plasmid

   #157882

   Purpose

   Expresses the RT-Abi-P2 defense system

   Depositor

   Feng Zhang

   Article

   Gao et al Science. 2020 Aug 28;369(6507):1077-1084

   Insert

   RT-Abi-P2
   

   Expression

   Bacterial

   Available Since

   Dec. 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pGP-RSET-iGABASnFR2-6xHis

   Plasmid

   #218885

   Purpose

   Bacterial expression of improved GABA sensor (positive change in fluorescence)

   Depositor

   GENIE Project

   Article

   Kolb et al bioRxiv 2025.03.25.644953

   Insert

   iGABASnFR2
   

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   S99A F102Y F104Y L178S

   Promoter

   T7

   Available Since

   May 14, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. dCas9-CBP core

   Plasmid

   #179543

   Purpose

   encodes S. pyogenes dCas9 with c-terminal fusion of human CBP core (aa 1084-1701) driven by EF-1alpha promoter

   Depositor

   Isaac Hilton

   Article

   Wang et al Nucleic Acids Res. 2022 Aug 12;50(14):78

   Insert

   S. Pyogenes dCas9 with c-terminal human CBP core (aa 1084-1701) (CREBBP Human)
   

   Use

   Lentiviral

   Tags

   Flag Tag

   Expression

   Mammalian

   Mutation

   D10A; H840A in S.pyogenes Cas9

   Promoter

   EF-1alpha

   Available Since

   Feb. 18, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pDONR223_EGFR_WT

   Plasmid

   #81926

   Purpose

   Gateway Donor vector containing EGFR, part of the Target Accelerator Plasmid Collection.

   Depositor

   Jesse Boehm, William Hahn, David Root

   Article

   Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

   Insert

   EGFR (EGFR Human)
   

   Use

   Gateway entry vector

   Mutation

   WT

   Promoter

   None

   Available Since

   April 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pEGFP-C1 beta-catenin WT

   Plasmid

   #16071

   Depositor

   Erin Schuman

   Article

   Murase et al Neuron. 2002 Jul 3. 35(1):91-105.

   Insert

   beta-catenin (CTNNB1 Chicken)
   

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   *

   Available Since

   Nov. 2, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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8. pUB_smHA_KDM5B_MS2

   Plasmid

   #81085

   Purpose

   Translation reporter: Encodes KDM5B (1544 aa) fused to smHA (Spaghetti Monster HA: 10 copies of an epitope tag HA).

   Depositor

   Tim Stasevich

   Article

   Morisaki et al Science. 2016 Jun 17;352(6292):1425-9. d

   Insert

   smHA_KDM5B_24xMS2
   

   Expression

   Mammalian

   Available Since

   Sept. 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. pIG-601_NY-ESO-1_TCR_GFP

   Plasmid

   #207482

   Purpose

   This plasmid can be used to generate HDR templates for non-viral knockin into the TRAC locus of human T cells.

   Depositor

   Alexander Marson

   Article

   Blaeschke et al Cell. 2023 Sep 14;186(19):4216-4234.e33.

   Insert

   GFP, NY-ESO-1_TCR
   

   Use

   CRISPR

   Mutation

   Potential silent point mutation in GFP sequence

   Available Since

   Jan. 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. lenti-NLS-miRFP670nano-miRFP670

    Plasmid

    #216131

    Purpose

    Lentiviral vector for expressing a fusion of miRFP670nano and miRFP670 localizing to the nucleus.

    Depositor

    Stefan Kubicek

    Article

    Reicher et al Nat Cell Biol. 2024 May;26(5):745-756. d

    Insert

    EF1a-NuclearLocalizationSignal-miRFP670nano-miRFP670-WPRE
    

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    April 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. BirA His-tag pLEMO

    Plasmid

    #119817

    Purpose

    Express endogenous biotin ligase in E.coli

    Depositor

    Alexander Gabibov

    Article

    Lomakin et al Bioconjug Chem. 2019 Apr 25. doi: 10.102

    Insert

    Biotin ligase
    

    Tags

    6-Histidine-tag

    Expression

    Bacterial

    Available Since

    May 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. PX459-CDKN1A

    Plasmid

    #217457

    Purpose

    For CDKN1A (p21) knockout

    Depositor

    John Diffley

    Article

    Zeng et al Cell. 2023 Feb 2;186(3):528-542.e14. doi

    Insert

    CRISPR Cas9 guide for CDKN1A
    

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    April 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pTUB1:YFP-mAID-3HA, DHFR-TS:HXGPRT

    Plasmid

    #87259

    Purpose

    YFP-mAID-3HA fusion driven by a minimal TUB1 promoter with an HXGdrug selectable marker. The mAID CDS is from Arabidopsis thaliana auxin-responsive protein IAA17E66-S133

    Depositor

    David Sibley

    Article

    Brown et al MBio. 2017 May 2;8(3). pii: e00375-17. d

    Inserts

    YFP-mAID-3HA
    HXGPRT
    

    Use

    Toxoplasma expression

    Tags

    3HA and mAID

    Mutation

    Codon optimized for Toxoplasma gondii expression

    Promoter

    TgDHFR-TS 463 bp and TgTUB1 378 bp

    Available Since

    May 4, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. pEGFPN1-ATG9A

    Plasmid

    #198529

    Purpose

    Expression of ATG9A-GFP fusion protein in mammalian cells

    Depositor

    Juan Bonifacino

    Article

    Mattera et al Proc Natl Acad Sci U S A. 2017 Dec 12;11

    Insert

    ATG9A (ATG9A Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    silent mutations in codons 4 and 775

    Promoter

    CMV

    Available Since

    March 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAAV_hSyn1_nLightG

    Plasmid

    #187179

    Purpose

    Expresses green norepinephrine indicator nLightG in neuronal cells

    Depositor

    Tommaso Patriarchi

    Article

    Kagiampaki et al Nat Methods. 2023 Sep;20(9):1426-1436. d

    Has Service

    AAV9

    Insert

    nLightG
    

    Use

    AAV

    Tags

    Flag tag

    Promoter

    human Synapsin-1

    Available Since

    July 31, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. GST-AR-624-919

    Plasmid

    #104200

    Purpose

    Expresses human AR-624-929 ligand binding domain in pGEX-5X-1 as GST fusion protein

    Depositor

    Elizabeth Wilson

    Article

    He et al Mol Cell Biol. 2003 Mar;23(6):2135-50.

    Insert

    human androgen receptor aa 624-919 (AR Human)
    

    Tags

    GST

    Expression

    Bacterial

    Available Since

    Dec. 6, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. TRE-Lepr_FL-IRES-eGFP

    Plasmid

    #196635

    Purpose

    Doxycycline inducible expression of full length mouse Lepr with eGFP marker

    Depositor

    Elaine Fuchs

    Article

    Yuan et al Nature. 2022 Nov 30. doi: 10.1038/s41586

    Insert

    Lepr (Lepr Mouse)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    TRE

    Available Since

    Feb. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. Caspase-Reporter

    Plasmid

    #243673

    Purpose

    Fluorescent Reporter for Caspase 3/7 activity. Contains DEVD sequence.

    Depositor

    Matthias Wirth

    Article

    Selcen et al Cell Death Discov, 11, 368, 06 August 20

    Insert

    Split-GFP with DEVD sequence and T2A mCherry
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    Sept. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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19. 7TGC

    Plasmid

    #24304

    Depositor

    Roel Nusse

    Article

    Fuerer et al PLoS One. 2010 . 5(2):e9370.

    Insert

    7xTcf-eGFP // SV40-mCherry
    

    Use

    Lentiviral

    Mutation

    EGFP is GFP with S65T and F64L mutations

    Available Since

    April 5, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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20. mCherry-Cytb5

    Plasmid

    #182579

    Purpose

    Expresses mCherry targeted to the ER via the CytB5RR tail anchor sequence

    Depositor

    Uri Manor

    Article

    Schiavon et al Nat Methods. 2020 Sep;17(9):917-921. doi

    Insert

    mCherry-Cytb5
    

    Tags

    Cytb5

    Expression

    Mammalian

    Available Since

    April 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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