164,826 results

1. pLVX-TetOne-zeo_3xHA-TurboID-NLS

   Plasmid

   #200022

   Purpose

   For lentiviral transduction of 3xHA-TurboID-NLS under control of a doxycyline-inducible promoter

   Depositor

   Britt Glaunsinger

   Article

   McCollum et al PLoS Pathog. 2023 Apr 17;19(4):e1011163.

   Insert

   3xHA-TurboID-NLS
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   TRE3Gs

   Available Since

   July 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pHR-EF1Alpha-Tet-on 3G

   Plasmid

   #118592

   Purpose

   Expresses Tet-on 3G from the EF1Alpha promoter

   Depositor

   Stanley Qi

   Article

   Liu et al Cell Stem Cell. 2018 Sep 28. pii: S1934-

   Insert

   Tet-on 3G
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1Alpha

   Available Since

   Nov. 29, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pRF-SrpR

   Plasmid

   #49372

   Depositor

   Christopher Voigt

   Article

   Stanton et al Nat Chem Biol. 2013 Dec 8. doi: 10.1038/

   Insert

   SrpR repressor
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Jan. 7, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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4. pBMN HA-NDP52

   Plasmid

   #199307

   Purpose

   For expression of the mammalian NDP52 tagged with HA

   Depositor

   Michael Lazarou

   Article

   Nguyen et al Mol Cell. 2023 May 18;83(10):1693-1709.e

   Insert

   NDP52 (CALCOCO2 Human)
   

   Use

   Retroviral

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   May 23, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pKM491-Flag-His

   Plasmid

   #109282

   Purpose

   ORBIT integrating plasmid for C-terminal tagging with FLAG-His tag

   Depositor

   Kenan Murphy

   Article

   Murphy et al MBio. 2018 Dec 11;9(6). pii: mBio.01467-

   Insert

   attB-Flag-His
   

   Use

   Orbit integrating plasmid

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   May 15, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. LV-TRE-WT human MyoD-T2A-dsRedExpress2

   Plasmid

   #60628

   Purpose

   Expresses WT human MyoD and dsRedExpress2 in response to doxycycline

   Depositor

   Charles Gersbach

   Article

   Kabadi et al ACS Synth Biol. 2014 Dec 10.

   Insert

   VP64 mouse MyoD (MYOD1 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   TRE

   Available Since

   Dec. 11, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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7. pnCS_SEPT6_SEPT7-TEV-Strep

   Plasmid

   #174499

   Purpose

   bacterial co-expression of human SEPT6 and of human SEPT7

   Depositor

   Manos Mavrakis

   Article

   Iv et al J Cell Sci. 2021 Jul 8. pii: 270842. doi

   Inserts

   SEPTIN6 (SEPTIN6 Human)
   SEPTIN7 (SEPTIN7 Human)
   

   Use

   Tags

   TEV-Strep

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Sept. 29, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAAV-hSyn-HA-hM3D(Gq)-IRES-mCitrine

   Plasmid

   #50463

   Purpose

   Gq-coupled hM3D-IRES-mCitrine under the control of human synapsin promoter

   Depositor

   Bryan Roth

   Insert

   hM3D(Gq)-IRES-mCitrine (CHRM3 Human)
   

   Use

   AAV

   Tags

   HA

   Expression

   Mutation

   See supplemental documents for DREADD mutations

   Promoter

   human Synapsin 1

   Available Since

   April 16, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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9. pMH-SFB-DYRK1A

   Plasmid

   #101770

   Purpose

   Mammalian expression of DYRK1A fusion protein

   Depositor

   Michael Huen

   Article

   Li et al Nucleic Acids Res. 2024 Jul 5:gkae574. d

   Insert

   DYRK1A (DYRK1A Human)
   

   Use

   Tags

   S peptide-flag-streptavidin binding peptide (SFB)

   Expression

   Mammalian

   Mutation

   siRNA-resistant allele

   Promoter

   Available Since

   Oct. 16, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pDEST-eGFP-C1-VPS35

    Plasmid

    #163622

    Purpose

    human VPS35 cDNA C-terminally tagged with eGFP

    Depositor

    Josef Kittler

    Article

    Halff et al Cell Rep. 2019 Nov 26;29(9):2599-2607.e6

    Insert

    human VPS35 (VPS35 Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Jan. 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pRSF-G1(4CNW)RS

    Plasmid

    #222871

    Purpose

    tRNA synthetase/tRNA pair for the in vivo incorporation of 4-Cyano-L-Tryptophan (4-CN-Trp) into proteins in E. coli in response to the amber (TAG) codon

    Depositor

    Thomas Huber

    Article

    Qianzhu et al Angew Chem Int Ed Engl. 2024 Dec 4:e2024

    Insert

    tRNA synthetase
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Dec. 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pBIG1a-PAF1C(CTR9/CDC73/PAF1/LEO1/WDR61)

    Plasmid

    #231733

    Purpose

    Protein expression in insect cells

    Depositor

    Jesper Svejstrup

    Article

    Lopez Martinez et al Mol Cell. 2025 May 15;85(10):1952-1967.e

    Inserts

    CTR9 (CTR9 Human)
    CDC73 (CDC73 Human)
    PAF1 (PAF1 Human)
    LEO1 (LEO1 Human)
    WDR61 (SKIC8 Human)
    

    Use

    Tags

    3C-Strep(II) and Flag-3C

    Expression

    Insect

    Mutation

    Promoter

    Available Since

    May 2, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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13. pFRT/TO/HIS/FLAG/HA-MYBBP1A

    Plasmid

    #38084

    Depositor

    Markus Landthaler

    Article

    Baltz et al Mol Cell. 2012 Jun 8;46(5):674-90.

    Insert

    MYBBP1A MYB binding protein (P160) 1a (MYBBP1A Human)
    

    Use

    Tags

    HIS/FLAG/HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Sept. 24, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCI-neo-HA-hUVRAG

    Plasmid

    #24290

    Depositor

    Noboru Mizushima

    Article

    Itakura et al Mol Biol Cell. 2008 Dec . 19(12):5360-72

    Insert

    UV irradiation resistance-associated gene (UVRAG Human)
    

    Use

    Tags

    3xHA

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    July 2, 2010

    Availability

    Academic Institutions and Nonprofits only

15. pSL21-VEX

    Plasmid

    #158230

    Purpose

    Retroviral expression plasmid of sgRNA with violet-excited fluorescent protein (VEX)

    Depositor

    Junwei Shi

    Article

    Chen et al Cell. 2021 Mar 4;184(5):1262-1280.e22. d

    Type

    Empty backbone

    Use

    CRISPR and Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6 promoter for crRNA expression and EFS promoter…

    Available Since

    Jan. 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pVS1-VIR2

    Plasmid

    #134745

    Purpose

    Virulence and replication helper

    Depositor

    Qi-Jun Chen

    Article

    Zhang et al Plant Physiol. 2019 Sep 26. pii: pp.19.0

    Insert

    vir gene cluster
    

    Use

    Vir gene expression

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Oct. 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pBluescript-minimal PB

    Plasmid

    #177151

    Purpose

    Minimal piggyBac ITR's in high copy number pBluescript backbone plasmid

    Depositor

    Mario Capecchi

    Type

    Empty backbone

    Use

    Piggybac

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Nov. 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. TFORF0459

    Plasmid

    #142266

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    CREB5 (CREB5 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available Since

    June 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCRISPRyl

    Plasmid

    #70007

    Purpose

    CRISPR/Cas9 vector for Yarrowia lipolytica, with AvrII site for sgRNA insertion

    Depositor

    Ian Wheeldon

    Article

    Schwartz et al ACS Synth Biol. 2015 Dec 29.

    Inserts

    Codon optimized Cas9 from S. pyogenes
    sgRNA expression cassette
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    SCR1'-tRNA and UAS1B8-TEF(136)

    Available Since

    Feb. 16, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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20. p2T-CMV-miniSdd6-BE4max-BlastR

    Plasmid

    #204850

    Purpose

    For cytosine base editing using miniSdd6 in mammalian cells

    Depositor

    Caixia Gao

    Article

    Huang et al Cell. 2023 Jul 20;186(15):3182-3195.e14.

    Insert

    miniSdd6-nSpCas9(D10A)-UGI
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    D10A in SpCas9

    Promoter

    CMV, SV40, EM7

    Available Since

    Aug. 17, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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