164,826 results

1. pX330-SpCas9-NG

   Plasmid

   #117919

   Purpose

   Expresses 3xFLAG-NLS-SpCas9-NG-NLS in mammalian cells.

   Depositor

   Osamu Nureki

   Article

   Nishimasu et al Science. 2018 Aug 30. pii: science.aas91

   Insert

   SpCas9-NG (L1111R/D1135V/G1218R/E1219F/A1322R/R1335V/T1337R)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Dec. 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLEX_306

   Plasmid

   #41391

   Purpose

   Constitutive lentiviral expression, SV40-puro; PGK-gateway-V5 tag

   Depositor

   David Root

   Type

   Empty backbone

   Use

   Lentiviral; Gateway destination vector

   Tags

   V5

   Expression

   Mammalian

   Mutation

   Promoter

   PGK

   Available Since

   Nov. 30, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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3. pDONR223-DCAMKL2

   Plasmid

   #23604

   Depositor

   David Root, William Hahn

   Article

   Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

   Insert

   DCAMKL2 (DCLK2 Human)
   

   Use

   Gateway donor vector

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   July 30, 2010

   Availability

   Academic Institutions and Nonprofits only

4. pJC49

   Plasmid

   #133060

   Purpose

   Lentiviral vector with a (Ubiquitous Chromatin Opening Element) UCOE upstream of a CAG promoter constitutively expressing a single insert containing the Tau.K18(P301L/V337M)-mScarlet-I fusion protein.

   Depositor

   Martin Kampmann

   Article

   Chen et al J Biol Chem. 2019 Oct 2. pii: RA119.0094

   Insert

   Tau.K18(LM)
   

   Use

   Lentiviral

   Tags

   mScarletI

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available Since

   Feb. 11, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pPPED

   Plasmid

   #162468

   Purpose

   Destination Expression vector for dicot plant prime editing

   Depositor

   Adam Bogdanove

   Article

   Wang et al Front Genome Ed. 2021 Mar 4;3:617553. do

   Inserts

   Cas9H840A
   attR gateway destination cassette
   

   Use

   Tags

   MLV reverse transcriptase

   Expression

   Plant

   Mutation

   Change histidine 840 to Alanine based sequence o…

   Promoter

   2x35S

   Available Since

   Dec. 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. PA-mCherry1-H2B-C-10

   Plasmid

   #57181

   Purpose

   Localization: Histones/Nucleus, Excitation: 400 / 504, Emission: 515 / 517

   Depositor

   Michael Davidson

   Insert

   H2B (H2BC11 Human)
   

   Use

   Tags

   PA-mCherry1

   Expression

   Mammalian

   Mutation

   D26G and V119I in H2B

   Promoter

   CMV

   Available Since

   Jan. 13, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. Geminin-mStrawberry reporter

   Plasmid

   #158690

   Purpose

   This plasmid is a Geminin cell cycle reporter. It has CMV promoter driving the expression of Geminin fused to mStrawberry-pGK-BSD

   Depositor

   Ravid Straussman

   Article

   Kaufman et al Nat Commun. 2022 May 18;13(1):2725. doi:

   Insert

   mStrawberry
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   May 18, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCS2+ phiC31 integrase

   Plasmid

   #216237

   Purpose

   For the overexpression of phiC31 integrase

   Depositor

   Hyunju Ro

   Article

   Hong et al Mol Ther Methods Clin Dev. 2024 Feb 2;32

   Insert

   integrase from phage φC31
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV IE94

   Available Since

   May 28, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pDONR221_MFSD14A

   Plasmid

   #131869

   Purpose

   Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

   Depositor

   RESOLUTE Consortium, Giulio Superti-Furga

   Article

   Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

   Insert

   MFSD14A (HIAT1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Oct. 9, 2019

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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10. 6 x DBE-luc

    Plasmid

    #153160

    Purpose

    Luciferase reporter plasmid for FOXTF

    Depositor

    Stefan Koch

    Article

    Moparthi et al Differentiation. 2020 Sep - Oct;115:30-3

    Insert

    luciferase
    

    Use

    Luciferase

    Tags

    Expression

    Mutation

    Promoter

    minP

    Available Since

    Aug. 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pHAGE Flag HA MPG

    Plasmid

    #237804

    Purpose

    Lentiviral vector expressing WT MPG

    Depositor

    David Pellman

    Article

    Tang et al Nature. 2022 Jun;606(7916):930-936. doi:

    Insert

    MPG (MPG Human)
    

    Use

    Tags

    Flag 3HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    April 24, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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12. Tol2-ngn1-Trpa1b-2A-mCherry

    Plasmid

    #106425

    Purpose

    For expressing Trpa1b-2A-mCherry in zebrafish Rohon-Beard neurons.

    Depositor

    Randall Peterson

    Article

    Lam et al Sci Rep. 2017 Sep 19;7(1):11839. doi: 10

    Inserts

    cmlc2 promoter
    Trpa1b (trpa1b Zebrafish)
    

    Use

    Gateway

    Tags

    2A-mCherry

    Expression

    Mutation

    Promoter

    Available Since

    April 2, 2018

    Availability

    Academic Institutions and Nonprofits only

13. pGL2- Empty control -SV40-Luc

    Plasmid

    #26280

    Purpose

    Addgene next-generation sequencing identified numerous modifications from the indicated plasmid backbone. Please see the sequences link for additional information.

    Depositor

    Rudolf Jaenisch

    Article

    Hanna et al Proc Natl Acad Sci U S A. 2010 May 18. 1

    Type

    Empty backbone

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Oct. 20, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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14. plRL61

    Plasmid

    #163633

    Purpose

    Optimized M. smegmatis CRISPRi plasmid; contains an L5 attP region (no L5 Integrase) that allows for integration when co-transformed with an L5 Integrase delivery plasmid (e.g. plRL19, ID #163634).

    Depositor

    Jeremy Rock

    Article

    Bosch et al Cell. 2021 Jul 16. pii: S0092-8674(21)00

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Tet-On

    Available Since

    Aug. 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. MXS_PGK::ZeoR-bGHpA

    Plasmid

    #62442

    Purpose

    MXS_chaining vector with PGK::ZeoR-bGHpA

    Depositor

    Pierre Neveu

    Article

    Sladitschek et al PLoS One. 2015 Apr 24;10(4):e0124958. do

    Insert

    resistance cassette against Zeocin with PGK Promoter
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    May 8, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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16. NP-11-64-1

    Plasmid

    #234359

    Purpose

    Expressing rubisco and prk in Δrpi

    Depositor

    David Savage

    Article

    Prywes et al Nature. 2025 Jan 22. doi: 10.1038/s41586

    Insert

    R.rubrum rubisco
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Ptac

    Available Since

    April 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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17. Parkin

    Plasmid

    #187897

    Purpose

    Mammalian cell expression of untagged Parkin

    Depositor

    Erika Holzbaur

    Article

    Harding et al Mol Cell. 2023 Sep 7;83(17):3188-3204.e7

    Insert

    PRKN (PRKN Human)
    

    Use

    Tags

    None

    Expression

    Mammalian

    Mutation

    None

    Promoter

    CMV

    Available Since

    April 2, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pKL2351

    Plasmid

    #199721

    Purpose

    A pVS1-based T-DNA binary vector and destination vector for Gateway cloning.

    Depositor

    Kan Wang

    Article

    Lee et al Plant Physiol. 2023 Apr 18:kiad231. doi:

    Insert

    NPTII
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available Since

    July 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pTBNde(min)

    Plasmid

    #15125

    Depositor

    Franco Pagani

    Article

    Pagani et al Hum Mol Genet. 2003 May 15. 12(10):1111-

    Insert

    alpha-globin EDB fibronectin minigene (FN1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    alpha-globin

    Available Since

    March 5, 2012

    Availability

    Academic Institutions and Nonprofits only

20. pCMV-LMO-IRES-EGFP

    Plasmid

    #178309

    Purpose

    Express the bacterial lactate 2-monooxygenase (LMO) enzyme in mammalian cells

    Depositor

    Sergey Kasparov

    Article

    Vaccari Cardoso et al Brain Sci. 2021 Aug 10;11(8). pii: brain

    Insert

    Lactate 2-monooxygenase
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Jan. 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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