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dCas-hHDAC3-R265P
(Plasmid #103786)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 103786 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    dCas9-HDAC3-R265P
  • Alt name
    histone deacetylase 3
  • Species
    H. sapiens (human), Synthetic; S. pyogenes
  • Mutation
    Changed arginine 265 to proline
  • GenBank ID
    NM_003883
  • Entrez Gene
    HDAC3 (a.k.a. HD3, KDAC3, RPD3, RPD3-2)
  • Promoter EF1A

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site BsrGI (not destroyed)
  • 5′ sequencing primer GTTTGGATCTTGGTTCATTCTCAAGCCTCAG
  • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    human HDAC3 cDNA from Addgene plasmid HDAC3-Flag (#13819) plenti vector containing dCas9_2A_Blast from Addgene plasmid (#61425)
  • Terms and Licenses

Depositor Comments

The stop codon present after the HDAC3 open reading frame may interfere with expression of the blasticidin resistance cassette. Blasticidin selection has not been verified with this plasmid

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    dCas-hHDAC3-R265P was a gift from Zhaolan Zhou (Addgene plasmid # 103786 ; http://n2t.net/addgene:103786 ; RRID:Addgene_103786)
  • For your References section:

    Locus-specific histone deacetylation using a synthetic CRISPR-Cas9-based HDAC. Kwon DY, Zhao YT, Lamonica JM, Zhou Z. Nat Commun. 2017 May 12;8:15315. doi: 10.1038/ncomms15315. 10.1038/ncomms15315 PubMed 28497787