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Primate immunodeficiency virus proteins Vpx and Vpr counteract transcriptional repression of proviruses by the HUSH complex.
Yurkovetskiy L, Guney MH, Kim K, Goh SL, McCauley S, Dauphin A, Diehl WE, Luban J
Nat Microbiol. 2018 Dec;3(12):1354-1361. doi: 10.1038/s41564-018-0256-x. Epub 2018 Oct 8.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
115803pscALPS gag-gfp BlastiExpress gag-gfp fusion driven by SFFV promoter
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115804pscALPS gfp BlastiExpress gfp driven by SFFV promoter
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115805pecALPS gag-gfp BlastiExpress gag-gfp fusion driven by EIF1a promoter
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115806ptcALPS gag-gfp BlastiExpress gag-gfp fusion driven by TK promoter
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115807pscALPS gag-gfp/deltavpxSFFV promoter expresses gag-gfp fusion with no ORF after CypA promoter
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115808pscALPS gag-gfp/vpxSFFV promoter expresses gag-gfp fusion with CypA promoter driving expression of SIVMAC251 vpx
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115809HIV-1 LTR-gfpHIV-1 LTR driven reporter vector that retains complete LTRs, tat, and rev, but has a frameshift mutation in env, an ngfr reporter gene in place of nef, and gfp in place of gag, pol, vif, and vpr
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115810pscALPS- SIV-MAC251 vpx Encodes codon optimized 3xFLAG-VpxMAC251 and puromycin resistance protein
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115811pscALPS SIV-MND2 vpxEncodes codon optimized 3xFLAG-SIVMND2 Vpx (AY159322) and puromycin resistance protein
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115812pscALPS-SIV-RCM vpxEncodes codon optimized 3xFLAG-SIVRCM Vpx (AF349680) and puromycin resistance protein
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115813pscALPS-SIV-MNE vpxEncodes codon optimized 3xFLAG-SIVMNE Vpx (U79412) and puromycin resistance protein
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115814pscALPS-SIV-DRL vpxEncodes codon optimized 3xFLAG-SIVDRL Vpx (KM378563) and puromycin resistance protein
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115815pscALPS-SIV-AGI vpxEncodes codon optimized 3xFLAG-SIVAGI Vpx (HM803690) and puromycin resistance protein
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115816pscALPS- HIV2-ROD vpxEncodes codon optimized 3xFLAG-HIV2ROD Vpx (M15390) and puromycin resistance protein
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115817pscALPS-SIV-MND1 vprEncodes codon optimized 3xFLAG-SIVMND1 Vpr (M27470) and puromycin resistance protein
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115818pscALPS-SIV-LST vprEncodes codon optimized 3xFLAG-SIVLST Vpr (AF188116) and puromycin resistance protein
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115819pscALPS-SIV-AGM-TAN1 vprEncodes codon optimized 3xFLAG-SIVAGM Vpr (TAN1) (U58991) with repaired premature stop codon and puromycin resistance protein
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115820pscALPS-SIV-TAL vprEncodes codon optimized 3xFLAG-SIVTAL Vpr (AM182197) and puromycin resistance protein
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115821pscALPS-SIV-SYK vprEncodes codon optimized 3xFLAG-SIVSYK Vpr (L06042) and puromycin resistance protein
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115822pscALPS-SIV-SAB-92018 vprEncodes codon optimized 3xFLAG-SIVSAB Vpr (HQ378594) and puromycin resistance protein
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115823pscALPS-SIV-DEB vprEncodes codon optimized 3xFLAG-SIVDEB Vpr (FJ919724) and puromycin resistance protein
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115824pscALPS-SIV-ASC vprEncodes codon optimized 3xFLAG-SIVASC Vpr (KJ461715) and puromycin resistance protein
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115825pscALPS-SIV-AGM-VER9063 vprEncodes codon optimized 3xFLAG-SIVAGM Vpr (Ver 9063) (L40990) and Puro resistance marker
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115826pscALPS-SIV-AGM-VERAGM3 vprEncodes codon optimized 3xFLAG-SIVAGM Vpr (Ver AGM3) (M30931) and puromycin resistance protein
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115827pscALPS-SIV-GRV vprEncodes codon optimized 3xFLAG-SIVGRV Vpr (M66437) and puromycin resistance protein
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115828pscALPS-SIV-AGM-MAL vprEncodes codon optimized 3xFLAG-SIVAGM Vpr (MAL_ZMB) (LC114462) and puromycin resistance protein
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115829pscALPS-SIV-DEN vprEncodes codon optimized 3xFLAG SIVDEN Vpr (AJ580407) and puromycin resistance protein
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115830pscALPS-SIV-GSN-CN71 vprEncodes codon optimized 3xFLAG SIVGSN-CN71 Vpr (AF468658) and puromycin resistance protein
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115831pscALPS-SIV-GSN-CN166 vprEncodes codon optimized 3xFLAG SIVGSN-CN166 Vpr (AF468659) and puromycin resistance protein
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115832pscALPS-SIV-SUN-K08 vprEncodes codon optimized 3xFLAG-SIVSUN Vpr (FR751162) and puromycin resistance protein
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115833pscALPS-SIV-CPZ-TAN3 vprEncodes codon optimized 3xFLAG-SIVCPZ-TAN3 Vpr (DQ374658) and puromycin resistance protein
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115834pscALPS-SIV-CPZ-LB7 vprEncodes codon optimized 3xFLAG-SIVCPZ-LB7 Vpr (DQ373064) and puromycin resistance protein
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115835pscALPS-SIV-GOR -vprEncodes codon optimized 3xFLAG-SIVGOR Vpr (FJ424871) and puromycin resistance protein
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115836pscALPS-HIV-1 Group O-vprEncodes codon optimized 3xFLAG-HIV1 Group O Vpr (L20571) and puromycin resistance protein
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115837pscALPS-HIV-1 Group P-vprEncodes codon optimized 3xFLAG-HIV1 Group P Vpr (HQ179987) and puromycin resistance protein
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115838pscALPS-SIV-RCM-vprEncodes codon optimized 3xFLAG-SIVRCM Vpr (HM803689) and puromycin resistance protein
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115839pscALPS-SIV-WRC-vprEncodes codon optimized 3xFLAG-SIVWRC Vpr (AM713177) and puromycin resistance protein
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115840pscALPS-SIV-COL-BWC01 vprEncodes codon optimized 3xFLAG-SIVCOLVpr (KF214240) and puromycin resistance protein
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115841pscALPS-SIV-MUS2-1246-vprEncodes codon optimized 3xFLAG-SIVMUS21246 Vpr (EF070329) and puromycin resistance protein
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115842pcDNA3.1 FLAG-SIV-MAC251-vpxEncodes codon optimized FLAG tagged SIVMAC251-Vpx
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115843pcDNA3.1 FLAG- SIV-MAC251-vpx-Q76AEncodes codon optimized FLAG tagged SIVMAC251-Vpx-Q76A mutant
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115844pcDNA3.1 FLAG-SIV-RCM-vpxEncodes codon optimized FLAG tagged SIVRCM-Vpx
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115845pcDNA3.1 HA-FAM208AEncodes codon optimized HA tagged FAM208A
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115846pAPM-D4- miR30-L1221Control shRNA KD vector
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115847pAPM-D4 miR30-AGO1 ts3AGO1 knockdown
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115848pAPM-D4 miR30-HDAC1 ts2HDAC1 knockdown
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115849pAPM-D4 miR30-HDAC1 ts3HDAC1 knockdown
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115850pAPM-D4 miR30-AGO1 ts1AGO1 knockdown
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115851pAPM-D4 miR30-AGO1 ts2AGO1 knockdown
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115852pAPM-D4 miR30-HDAC1 ts1HDAC1 knockdown
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115853pAPM-D4 miR30-AGO2 ts1AGO2 knockdown
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115854pAPM-D4 miR30-AGO2 ts2AGO2 knockdown
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115855pAPM-D4 miR30-AGO2 ts3AGO2 knockdown
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115856pAPM-D4 miR30-AGO3 ts1AGO3 knockdown
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115857pAPM-D4 miR30-AGO3 ts2AGO3 knockdown
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115858pAPM-D4 miR30-AGO3 ts3AGO3 knockdown
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115859pAPM-D4 miR30-SETDB1 ts1SETDB1 knockdown
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115860pAPM-D4 miR30-SETDB1 ts2SETDB1 knockdown
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115861pAPM-D4 miR30-SETDB1 ts3SETDB1 knockdown
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115862pAPM-D4 miR30-TRIM28 ts1TRIM28 knockdown
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115864pAPM-D4 miR30-TRIM28 ts3TRIM28 knockdown
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115865pAPM-D4 miR30-DNMT3a ts1DNMT3a knockdown
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115866pAPM-D4 miR30-DNMT3a ts2DNMT3a knockdown
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115867pAPM-D4 miR30-DNMT3a ts3DNMT3a knockdown
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115868pAPM-D4 miR30-PIWIL2 ts1PIWIL2 knockdown
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115869pAPM-D4 miR30-PIWIL2 ts3PIWIL2 knockdown
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115870pAPM-D4 miR30-PIWIL2 ts2PIWIL2 knockdown
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115871pAPM-D4 miR30-FAM208A ts1FAM208a knockdown
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115872pAPM-D4 miR30-FAM208A ts2FAM208a knockdown
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115873pAPM-D4 miR30-FAM208A ts3FAM208a knockdown
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115874pAPM-D4 miR30-MPHOSPH8 ts1MPHOSPH8 knockdown
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115875pAPM-D4 miR30-MPHOSPH8 ts2MPHOSPH8 knockdown
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115876pAPM-D4 miR30-MPHOSPH8 ts3MPHOSPH8 knockdown
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115877pAPM-D4 miR30-PPHLN1 ts1PPHLN1 knockdown
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115878pAPM-D4 miR30-PPHLN1 ts2PPHLN1 knockdown
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115879pAPM-D4 miR30-PPHLN1 ts3PPHLN1 knockdown
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115880pAPM-D4 miR30-SUV39h1 ts1SUV39h1 knockdown
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115881pAPM-D4 miR30-SUV39h1 ts2SUV39h1 knockdown
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115882pAPM-D4 miR30-SUV39h1 ts3SUV39h1 knockdown
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115883pAPM-D4 miR30-SUV39h2 ts1SUV39h2 knockdown
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115884pAPM-D4 miR30-SUV39h2 ts2SUV39h2 knockdown
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115885pAPM-D4 miR30-SUV39h2 ts3SUV39h2 knockdown
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115886pAPM-D4 miR30-MORC2 ts1MORC2 knockdown
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115887pAPM-D4 miR30-MORC2 ts2MORC2 knockdown
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115888pAPM-D4 miR30-MORC2 ts3MORC2 knockdown
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128504pscALPS_puroEncodes puromycin resistance protein
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128505pAPM D4 BsmBI Myd88 stufferpAPM knockdown vector with Myd88 stuffer sequence
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