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An improved secretion signal enhances the secretion of model proteins from Pichia pastoris.
Barrero JJ, Casler JC, Valero F, Ferrer P, Glick BS
Microb Cell Fact. 2018 Oct 12;17(1):161. doi: 10.1186/s12934-018-1009-5.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
117658pPICZ-pre-pro-alphaf-E2-CrimsonTest intracellular localisation of E2-Crimson and study the secretion efficiency
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117660pPICZ-pre-pro-alphaf(I)-E2-CrimsonTest intracellular localisation of E2-Crimson and study the secretion efficiency
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117661pPICZ-pre-Ost1-alphaf(I)-E2-CrimsonTest intracellular localisation of E2-Crimson and study the secretion efficiency
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117662pPICZ-pOst1-pro-alphaf(MUT1)-E2-CrimsonTest intracellular localisation of E2-Crimson and study the secretion efficiency
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117663pPICZ-pOst1-pro-alphaf(MUT2)-E2-CrimsonTest intracellular localisation of E2-Crimson and study the secretion efficiency
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117664pPICZ-pre-pro-alphaf-Btl2Study secretion efficiency of Btl2
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117665pPICZ-pre-Ost1-pro-alphaf-Btl2Study secretion efficiency of Btl2
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117667pPICZ-pre-Ost1-alphaf(I)-Btl2Study secretion efficiency of Btl2
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117668pKar2-msGFP-HDELLocalisation of Endoplasmic Reticulum
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117669Htb2-GFPColorize the nuclei
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Antibodies from Article