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CRISPR-Cas9 genome editing in human cells occurs via the Fanconi anemia pathway.
Richardson CD, Kazane KR, Feng SJ, Zelin E, Bray NL, Schafer AJ, Floor SN, Corn JE
Nat Genet. 2018 Aug;50(8):1132-1139. doi: 10.1038/s41588-018-0174-0. Epub 2018 Jul 27.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
111091pCR1063Expresses dCas9-KRAB tagged with mCherry: pHR-hEF1a-dCas9-mcherry-KRAB-mPGK-Hyg
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111092pCR1068BFP/puromycin marked gRNA vector. Contains sgRNA against GFP/BFP N-terminus.
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111093pCR1075Expresses eGFP donor in pUC19
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111094pCR1265lentiviral cDNA expression vector: plenti-x1-neo-linker-IRES-Thy1.1
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111096pCR1110Expresses BLM_+_91260645.23-P1P2 sgRNA in pCR1068
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111097pCR1161Expresses FANCA_-_89883045.23-P1P2 sgRNA in pCR1068
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111098pCR1162Expresses FANCA_-_89883048.23-P1P2 sgRNA in pCR1068
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111099pCR1163Expresses FANCD2_+_10068200.23-P1P2 sgRNA in pCR1068
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111100pCR1164Expresses FANCD2_-_10068140.23-P1P2 sgRNA in pCR1068
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111101pCR1277Expresses RAD51D_-_33446794.23-P1P2 sgRNA in pCR1068
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111102pCR1082Expresses FANCE_-_35420187.23-P1P2 sgRNA in pCR1068
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111103pCR1135Expresses FANCE_-_35420147.23-P1P2 sgRNA in pCR1068
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111104pCR1178Expresses FANCF_-_22647294.23-P1P2 sgRNA in pCR1068
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111105pCR1224Expresses FANCF_-_22647359.23-P1P2 sgRNA in pCR1068
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111106pCR1271Expresses FANCI_-_89787239.23-P1P2 sgRNA in pCR1068
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111107pCR1272Expresses FANCI_+_89787428.23-P1P2 sgRNA in pCR1068
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111109pCR1322Expresses FANCL_+_58468435.23-P1P2 sgRNA in pCR1068
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111110pCR1118Expresses HELQ_+_84376961.23-P1P2 sgRNA in pCR1068
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111111pCR1165Expresses HELQ_-_84376979.23-P1P2 sgRNA in pCR1068
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111112pCR1273Expresses RAD51B_-_68286555.23-P1P2 sgRNA in pCR1068
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111113pCR1274Expresses RAD51B_-_68286667.23-P1P2 sgRNA in pCR1068
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111114pCR1275Expresses RAD51C_+_56770199.23-P1P2 sgRNA in pCR1068
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111115pCR1276Expresses RAD51C_+_56769980.23-P1P2 sgRNA in pCR1068
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111116pCR1319Expresses UBE2T_+_202311076.23-P1P2 sgRNA in pCR1068
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111117pCR1320Expresses UBE2T_-_202311067.23-P1P2 sgRNA in pCR1068
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111118pCR1299Expresses USP1_+_62903191.23-P1P2 sgRNA in pCR1068
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111119pCR1300Expresses USP1_+_62903144.23-P1P2 sgRNA in pCR1068
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111120pCR1324Expresses WDR48_+_39093886.23-P1P2 sgRNA in pCR1068
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111121pCR1278Expresses XRCC2_-_152373174.23-P1P2 sgRNA in pCR1068
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111122pCR1279Expresses XRCC2_+_152373143.23-P1P2 sgRNA in pCR1068
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111123pCR1280Expresses XRCC3_+_104181759.23-P1P2 sgRNA in pCR1068
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111124pCR1281Expresses XRCC3_+_104181786.23-P1P2 sgRNA in pCR1068
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111125pCR1323Expresses non-targeting_01224 sgRNA in pCR1068
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111126pCR1316Expresses FANCA.
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111128pCR1306Expresses FANCE.
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111129pCR1446Expresses FANCF
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111131pCR1326Expresses UBE2T.
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111132pCR1325Expresses USP1.
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111133pCR1307Expresses WDR48.
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