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Site-specific manipulation of Arabidopsis loci using CRISPR-Cas9 SunTag systems.
Papikian A, Liu W, Gallego-Bartolome J, Jacobsen SE
Nat Commun. 2019 Feb 13;10(1):729. doi: 10.1038/s41467-019-08736-7.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
115480pEG302 5aa SunTag VP64 nogCRISPR-Cas9 SunTag system to target VP64 to specific loci of interest (nog=no guide)
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115481pEG302 5aa SunTag VP64 gRNA4 (FWA)CRISPR-Cas9 SunTag system to target VP64 to the FWA locus with a single guide RNA
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115482pEG302 5aa SunTag VP64 gRNA17 (FWA)CRISPR-Cas9 SunTag system to target VP64 to the FWA locus with a single guide RNA
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1154835aa SunTag VP64 EVDCRISPR-Cas9 SunTag system to target VP64 to the EVD/ATR loci each with two guide RNAs
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1154845aa SunTag VP64 AP3CRISPR-Cas9 SunTag system to target VP64 to the AP3 locus with two guide RNAs
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1154855aa SunTag VP64 CLV3CRISPR-Cas9 SunTag system to target VP64 to the CLV3 locus with two guide RNAs
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115486pEG302 22aa SunTag NtDRMcd gRNA4 (FWA)CRISPR-Cas9 SunTag system to target NtDRMcd to the FWA locus with a single guide RNA
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115487pEG302 22aa SunTag NtDRMcd g4+g10+g18 (FWA)CRISPR Cas9 SunTag system to target NtDRMcd to the FWA locus with three guide RNAs
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115488pEG302 22aa SunTag NtDRMcd nogCRISPR-Cas9 SunTag system to target NtDRMcd to specific loci of interest (nog=no guide)
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115489pEG302 22aa SunTag NtDRMcd SUPCRISPR-Cas9 SunTag system to target NtDRMcd to the SUPERMAN locus with two guide RNAs
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115490pEG302 22aa SunTag NtDRMcd (no NLS) SUPCRISPR-Cas9 SunTag system to target NtDRMcd (without an NLS) to the SUPERMAN locus with two guide RNAs
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117168pEG302 22aa SunTag NtDRMcd (no NLS) g4+g10+g18 (FWA)CRISPR Cas9 SunTag system to target NtDRMcd (without an NLS) to the FWA locus with three guide RNAs
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119554pEG302 22aa SunTag NtDRMcd (noNLS) nogCRISPR-Cas9 SunTag system to target NtDRMcd (without an NLS) to specific loci of interest (nog=no guide)
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119672pEG302 5aa SunTag VP64 g4+g17 (FWA)CRISPR-Cas9 SunTag system to target VP64 to the FWA locus with two guide RNAs
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120249pEG302 22aa SunTag VP64 gRNA4 (FWA)CRISPR-Cas9 SunTag system to target VP64 to the FWA locus with a single guide RNA
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120250pEG302 22aa SunTag VP64 gRNA17 (FWA)CRISPR-Cas9 SunTag system to target VP64 to the FWA locus with a single guide RNA
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120251pEG302 22aa SunTag VP64 nogCRISPR-Cas9 SunTag system to target VP64 to specific loci of interest (nog=no guide)
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120252pEG302 22aa SunTag VP64 g4+g17CRISPR-Cas9 SunTag system to target VP64 to the FWA locus with two guide RNAs
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