qTAG: An adaptable CRISPR-based endogenous tagging protocol using optimized repair cassettes
Philip R, Sharma A, Matellan L, Erpf AC, Hsu W, Tkach JM, Wyatt HDM, Pelletier L
bioRxiv
(Link opens in a new window)
Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 207676 | qTAG-N-Blast-mNeon | Empty N-terminus donor cassette. Integrate homology arms to target the N-terminus insertion of a Blast-2A-mNeon cassette |
| 207677 | qTAG-N-Blast-moxGFP | Empty N-terminus donor cassette. Integrate homology arms to target the N-terminus insertion of a Blast-2A-moxGFP cassette |
| 207678 | qTAG-N-Blast-mScarlet | Empty N-terminus donor cassette. Integrate homology arms to target the N-terminus insertion of a Blast-2A-mScarlet cassette |
| 207679 | qTAG-N-Blast-sTagRFP | Empty N-terminus donor cassette. Integrate homology arms to target the N-terminus insertion of a Blast-2A-sTagRFP cassette |
| 207680 | qTAG-N-Blast-miRFP670nano3 | Empty N-terminus donor cassette. Integrate homology arms to target the N-terminus insertion of a Blast-2A-miRFP670nano3 cassette |
| 207681 | qTAG-N-Blast-miniTurbo | Empty N-terminus donor cassette. Integrate homology arms to target the N-terminus insertion of a Blast-2A-miniTurbo cassette |
| 207682 | qTAG-N-Blast-ultraID | Empty N-terminus donor cassette. Integrate homology arms to target the N-terminus insertion of a Blast-2A-ultraID cassette |
| 207683 | qTAG-N-Blast-dTAG | Empty N-terminus donor cassette. Integrate homology arms to target the N-terminus insertion of a Blast-2A-dTAG cassette |
| 207684 | qTAG-N-Blast-3xFLAG | Empty N-terminus donor cassette. Integrate homology arms to target the N-terminus insertion of a Blast-2A-3xFLAG cassette |
| 207685 | qTAG-N-Blast-3xHA | Empty N-terminus donor cassette. Integrate homology arms to target the N-terminus insertion of a Blast-2A-3xHA cassette |
| 207686 | qTAG-N-Blast-V5 | Empty N-terminus donor cassette. Integrate homology arms to target the N-terminus insertion of a Blast-2A-V5 cassette |
| 207687 | qTAG-N-Puro-mNeon | Empty N-terminus donor cassette. Integrate homology arms to target the N-terminus insertion of a Puro-2A-mNeon cassette |
| 207688 | qTAG-N-Puro-moxGFP | Empty N-terminus donor cassette. Integrate homology arms to target the N-terminus insertion of a Puro-2A-moxGFP cassette |
| 207689 | qTAG-N-Puro-mScarlet | Empty N-terminus donor cassette. Integrate homology arms to target the N-terminus insertion of a Puro-2A-mScarlet cassette |
| 207690 | qTAG-N-Puro-sTagRFP | Empty N-terminus donor cassette. Integrate homology arms to target the N-terminus insertion of a Puro-2A-sTagRFP cassette |
| 207691 | qTAG-N-Puro-miRFP670nano3 | Empty N-terminus donor cassette. Integrate homology arms to target the N-terminus insertion of a Puro-2A-miRFP670nano3 cassette |
| 207692 | qTAG-N-Puro-miniTurbo | Empty N-terminus donor cassette. Integrate homology arms to target the N-terminus insertion of a Puro-2A-miniTurbo cassette |
| 207693 | qTAG-N-Puro-ultraID | Empty N-terminus donor cassette. Integrate homology arms to target the N-terminus insertion of a Puro-2A-ultraID cassette |
| 207694 | qTAG-N-Puro-dTAG | Empty N-terminus donor cassette. Integrate homology arms to target the N-terminus insertion of a Puro-2A-dTAG cassette |
| 207695 | qTAG-N-Puro-3xFLAG | Empty N-terminus donor cassette. Integrate homology arms to target the N-terminus insertion of a Puro-2A-3xFLAG cassette |
| 207696 | qTAG-N-Puro-3xHA | Empty N-terminus donor cassette. Integrate homology arms to target the N-terminus insertion of a Puro-2A-3xHA cassette |
| 207697 | qTAG-N-Puro-V5 | Empty N-terminus donor cassette. Integrate homology arms to target the N-terminus insertion of a Puro-2A-V5 cassette |
| 207698 | qTAG-N-Zeo-mNeon | Empty N-terminus donor cassette. Integrate homology arms to target the N-terminus insertion of a Zeo-2A-mNeon cassette |
| 207699 | qTAG-N-Zeo-moxGFP | Empty N-terminus donor cassette. Integrate homology arms to target the N-terminus insertion of a Zeo-2A-moxGFP cassette |
| 207700 | qTAG-N-Zeo-mScarlet | Empty N-terminus donor cassette. Integrate homology arms to target the N-terminus insertion of a Zeo-2A-mScarlet cassette |
| 207701 | qTAG-N-Zeo-sTagRFP | Empty N-terminus donor cassette. Integrate homology arms to target the N-terminus insertion of a Zeo-2A-sTagRFP cassette |
| 207702 | qTAG-N-Zeo-miRFP670nano3 | Empty N-terminus donor cassette. Integrate homology arms to target the N-terminus insertion of a Zeo-2A-miRFP670nano3 cassette |
| 207703 | qTAG-N-Zeo-miniTurbo | Empty N-terminus donor cassette. Integrate homology arms to target the N-terminus insertion of a Zeo-2A-miniTurbo cassette |
| 207704 | qTAG-N-Zeo-ultraID | Empty N-terminus donor cassette. Integrate homology arms to target the N-terminus insertion of a Zeo-2A-ultraID cassette |
| 207705 | qTAG-N-Zeo-dTAG | Empty N-terminus donor cassette. Integrate homology arms to target the N-terminus insertion of a Zeo-2A-dTAG cassette |
| 207706 | qTAG-N-Zeo-3xFLAG | Empty N-terminus donor cassette. Integrate homology arms to target the N-terminus insertion of a Zeo-2A-3xFLAG cassette |
| 207707 | qTAG-N-Zeo-3xHA | Empty N-terminus donor cassette. Integrate homology arms to target the N-terminus insertion of a Zeo-2A-3xHA cassette |
| 207708 | qTAG-N-Zeo-V5 | Empty N-terminus donor cassette. Integrate homology arms to target the N-terminus insertion of a Zeo-2A-V5 cassette |
| 207709 | qTAG-C-mNeon-Blast | Empty C-terminus donor cassette. Integrate homology arms to target the C-terminus insertion of a mNeon-2A-Blast cassette |
| 207710 | qTAG-C-moxGFP-Blast | Empty C-terminus donor cassette. Integrate homology arms to target the C-terminus insertion of a moxGFP-2A-Blast cassette |
| 207711 | qTAG-C-mScarlet-Blast | Empty C-terminus donor cassette. Integrate homology arms to target the C-terminus insertion of a mScarlet-2A-Blast cassette |
| 207712 | qTAG-C-sTagRFP-Blast | Empty C-terminus donor cassette. Integrate homology arms to target the C-terminus insertion of a sTagRFP-2A-Blast cassette |
| 207713 | qTAG-C-miRFP670nano3-Blast | Empty C-terminus donor cassette. Integrate homology arms to target the C-terminus insertion of a miRFP670nano3-2A-Blast cassette |
| 207714 | qTAG-C-miniTurbo-Blast | Empty C-terminus donor cassette. Integrate homology arms to target the C-terminus insertion of a miniTurbo-2A-Blast cassette |
| 207715 | qTAG-C-ultraID-Blast | Empty C-terminus donor cassette. Integrate homology arms to target the C-terminus insertion of a ultraID-2A-Blast cassette |
| 207716 | qTAG-C-dTAG-Blast | Empty C-terminus donor cassette. Integrate homology arms to target the C-terminus insertion of a dTAG-2A-Blast cassette |
| 207717 | qTAG-C-3xFLAG-Blast | Empty C-terminus donor cassette. Integrate homology arms to target the C-terminus insertion of a 3xFLAG-2A-Blast cassette |
| 207718 | qTAG-C-3xHA-Blast | Empty C-terminus donor cassette. Integrate homology arms to target the C-terminus insertion of a 3xHA-2A-Blast cassette |
| 207719 | qTAG-C-V5-Blast | Empty C-terminus donor cassette. Integrate homology arms to target the C-terminus insertion of a V5-2A-Blast cassette |
| 207720 | qTAG-C-mNeon-Puro | Empty C-terminus donor cassette. Integrate homology arms to target the C-terminus insertion of a mNeon-2A-Puro cassette |
| 207721 | qTAG-C-moxGFP-Puro | Empty C-terminus donor cassette. Integrate homology arms to target the C-terminus insertion of a moxGFP-2A-Puro cassette |
| 207722 | qTAG-C-mScarlet-Puro | Empty C-terminus donor cassette. Integrate homology arms to target the C-terminus insertion of a mScarlet-2A-Puro cassette |
| 207724 | qTAG-C-miRFP670nano3-Puro | Empty C-terminus donor cassette. Integrate homology arms to target the C-terminus insertion of a miRFP670nano3-2A-Puro cassette |
| 207725 | qTAG-C-miniTurbo-Puro | Empty C-terminus donor cassette. Integrate homology arms to target the C-terminus insertion of a miniTurbo-2A-Puro cassette |
| 207727 | qTAG-C-dTAG-Puro | Empty C-terminus donor cassette. Integrate homology arms to target the C-terminus insertion of a dTAG-2A-Puro cassette |
| 207728 | qTAG-C-3xFLAG-Puro | Empty C-terminus donor cassette. Integrate homology arms to target the C-terminus insertion of a 3xFLAG-2A-Puro cassette |
| 207729 | qTAG-C-3xHA-Puro | Empty C-terminus donor cassette. Integrate homology arms to target the C-terminus insertion of a 3xHA-2A-Puro cassette |
| 207730 | qTAG-C-V5-Puro | Empty C-terminus donor cassette. Integrate homology arms to target the C-terminus insertion of a V5-2A-Puro cassette |
| 207731 | qTAG-C-mNeon-Zeo | Empty C-terminus donor cassette. Integrate homology arms to target the C-terminus insertion of a mNeon-2A-Zeo cassette |
| 207732 | qTAG-C-moxGFP-Zeo | Empty C-terminus donor cassette. Integrate homology arms to target the C-terminus insertion of a moxGFP-2A-Zeo cassette |
| 207733 | qTAG-C-mScarlet-Zeo | Empty C-terminus donor cassette. Integrate homology arms to target the C-terminus insertion of a mScarlet-2A-Zeo cassette |
| 207735 | qTAG-C-miRFP670nano3-Zeo | Empty C-terminus donor cassette. Integrate homology arms to target the C-terminus insertion of a miRFP670nano3-2A-Zeo cassette |
| 207736 | qTAG-C-miniTurbo-Zeo | Empty C-terminus donor cassette. Integrate homology arms to target the C-terminus insertion of a miniTurbo-2A-Zeo cassette |
| 207737 | qTAG-C-ultraID-Zeo | Empty C-terminus donor cassette. Integrate homology arms to target the C-terminus insertion of a ultraID-2A-Zeo cassette |
| 207738 | qTAG-C-dTAG-Zeo | Empty C-terminus donor cassette. Integrate homology arms to target the C-terminus insertion of a dTAG-2A-Zeo cassette |
| 207739 | qTAG-C-3xFLAG-Zeo | Empty C-terminus donor cassette. Integrate homology arms to target the C-terminus insertion of a 3xFLAG-2A-Zeo cassette |
| 207740 | qTAG-C-3xHA-Zeo | Empty C-terminus donor cassette. Integrate homology arms to target the C-terminus insertion of a 3xHA-2A-Zeo cassette |
| 207741 | qTAG-C-V5-Zeo | Empty C-terminus donor cassette. Integrate homology arms to target the C-terminus insertion of a V5-2A-Zeo cassette |
| 207742 | pEF1α-Cre-PGK-Puro | Co-expression of the Cre recombinase and a puro selectable marker |
| 207743 | pEF1α-Cre-2A-Puro | Expression of the Cre recombinase linked to a puro selectable marker |
| 207744 | pEF1α-Cre-2A-moxGFP | Expression of the Cre recombinase linked to a moxGFP fluorescent marker |
| 207745 | pEF1α-Cre-2A-mScarlet | Expression of the Cre recombinase linked to a mScarlet fluorescent marker |
| 207746 | pEF1α-Cre-2A-mTagBFP2 | Expression of the Cre recombinase linked to a mTagBFP2 fluorescent marker |
| 207747 | pEF1α-Cre-2A-miRFP670nano3 | Expression of the Cre recombinase linked to a miRFP670nano3 fluorescent marker |
| 207748 | pX330-PITCh-ACTB | Expresses SpCas9, the PITCh gRNA, and a sgRNA targeting the N-terminus of ACTB for knock-in. |
| 207749 | qTAG-N-Solo-mNeon-ACTB | Donor template for mNeon insertion into the N-terminus of the ACTB locus for actin visualization. To be co-transfected with sgRNA plasmid px330-PITCh-ACTB Addgene #207748 |
| 207750 | qTAG-N-Solo-mScarlet-ACTB | Donor template for mScarlet insertion into the N-terminus of the ACTB locus for actin visualization. To be co-transfected with sgRNA plasmid px330-PITCh-ACTB Addgene #207748 |
| 207751 | qTAG-N-Blast-mNeon-ACTB | Donor template for Blast-2A-mNeon insertion into the N-terminus of the ACTB locus for actin visualization. To be co-transfected with sgRNA plasmid px330-PITCh-ACTB Addgene #207748 |
| 207752 | qTAG-N-Puro-mNeon-ACTB | Donor template for Puro-2A-mNeon insertion into the N-terminus of the ACTB locus for actin visualization. To be co-transfected with sgRNA plasmid px330-PITCh-ACTB Addgene #207748 |
| 207753 | qTAG-N-Blast-mScarlet-ACTB | Donor template for Blast-2A-mScarlet insertion into the N-terminus of the ACTB locus for actin visualization. To be co-transfected with sgRNA plasmid px330-PITCh-ACTB Addgene #207748 |
| 207754 | qTAG-N-Puro-mScarlet-ACTB | Donor template for Puro-2A-mScarlet insertion into the N-terminus of the ACTB locus for actin visualization. To be co-transfected with sgRNA plasmid px330-PITCh-ACTB Addgene #207748 |
| 207755 | pX330-PITCh-H2BC11 | Expresses SpCas9, the PITCh gRNA, and a sgRNA targeting the C-terminus of H2BC11 for knock-in. |
| 207756 | qTAG-C-Solo-mScarlet-H2BC11 | Donor template for mScarlet insertion into the C-terminus of the H2BC11 locus for nuclei visualization. To be co-transfected with sgRNA plasmid px330-PITCh-H2BC11 Addgene #207755 |
| 207757 | qTAG-C-moxGFP-Blast-H2BC11 | Donor template for moxGFP-2A-Blast insertion into the C-terminus of the H2BC11 locus for nuclei visualization. To be co-transfected with sgRNA plasmid px330-PITCh-H2BC11 Addgene #207755 |
| 207758 | qTAG-C-moxGFP-Puro-H2BC11 | Donor template for moxGFP-2A-Puro insertion into the C-terminus of the H2BC11 locus for nuclei visualization. To be co-transfected with sgRNA plasmid px330-PITCh-H2BC11 Addgene #207755 |
| 207759 | qTAG-C-moxGFP-Zeo-H2BC11 | Donor template for moxGFP-2A-Zeo insertion into the C-terminus of the H2BC11 locus for nuclei visualization. To be co-transfected with sgRNA plasmid px330-PITCh-H2BC11 Addgene #207755 |
| 207760 | qTAG-C-moxGFP-Puro-H2BC11-MMEJ | MMEJ donor template for moxGFP-2A-Puro insertion into the C-terminus of the H2BC11 locus for nuclei visualization. To be co-transfected with sgRNA plasmid px330-PITCh-H2BC11 Addgene #207755 |
| 207761 | qTAG-C-mScarlet-Puro-H2BC11 | Donor template for mScarlet-2A-Puro insertion into the C-terminus of the H2BC11 locus for nuclei visualization. To be co-transfected with sgRNA plasmid px330-PITCh-H2BC11 Addgene #207755 |
| 207762 | qTAG-C-miRFP670nano3-Blast-H2BC11 | Donor template for miRFP670nano3-2A-Blast insertion into the C-terminus of the H2BC11 locus for nuclei visualization. To be co-transfected with sgRNA plasmid px330-PITCh-H2BC11 Addgene #207755 |
| 207763 | pX330-PITCh-TUBA1B | Expresses SpCas9, the PITCh gRNA, and a sgRNA targeting the N-terminus of TUBA1B for knock-in. |
| 207764 | qTAG-N-Solo-mScarlet-TUBA1B | Donor template for mScarlet insertion into the N-terminus of the TUBA1B locus for tubulin visualization. To be co-transfected with sgRNA plasmid px330-PITCh-TUBA1B Addgene #207763 |
| 207765 | qTAG-N-Solo-sTagRFP-TUBA1B | Donor template for sTagRFP insertion into the N-terminus of the TUBA1B locus for tubulin visualization. To be co-transfected with sgRNA plasmid px330-PITCh-TUBA1B Addgene #207763 |
| 207766 | qTAG-N-Blast-moxGFP-TUBA1B | Donor template for Blast-2A-moxGFP insertion into the N-terminus of the TUBA1B locus for tubulin visualization. To be co-transfected with sgRNA plasmid px330-PITCh-TUBA1B Addgene #207763 |
| 207767 | qTAG-N-Puro-moxGFP-TUBA1B | Donor template for Puro-2A-moxGFP insertion into the N-terminus of the TUBA1B locus for tubulin visualization. To be co-transfected with sgRNA plasmid px330-PITCh-TUBA1B Addgene #207763 |
| 207768 | qTAG-N-Blast-sTagRFP-TUBA1B | Donor template for Blast-2A-sTagRFP insertion into the N-terminus of the TUBA1B locus for tubulin visualization. To be co-transfected with sgRNA plasmid px330-PITCh-TUBA1B Addgene #207763 |
| 207769 | qTAG-N-Puro-sTagRFP-TUBA1B | Donor template for Puro-2A-sTagRFP insertion into the N-terminus of the TUBA1B locus for tubulin visualization. To be co-transfected with sgRNA plasmid px330-PITCh-TUBA1B Addgene #207763 |
| 207770 | pX330-PITCh-LMNB1 | Expresses SpCas9, the PITCh gRNA, and a sgRNA targeting the N-terminus of LMNB1 for knock-in. |
| 207771 | qTAG-N-Solo-mScarlet-LMNB1 | Donor template for mScarlet insertion into the N-terminus of the LMNB1 locus for nuclear envelope visualization. To be co-transfected with sgRNA plasmid px330-PITCh-LMNB1 Addgene #207770 |
| 207772 | qTAG-N-Blast-moxGFP-LMNB1 | Donor template for Blast-2A-moxGFP insertion into the N-terminus of the LMNB1 locus for nuclear envelope visualization. To be co-transfected with sgRNA plasmid px330-PITCh-LMNB1 Addgene #207770 |
| 207773 | qTAG-N-Puro-moxGFP-LMNB1 | Donor template for Puro-2A-moxGFP insertion into the N-terminus of the LMNB1 locus for nuclear envelope visualization. To be co-transfected with sgRNA plasmid px330-PITCh-LMNB1 Addgene #207770 |
| 207774 | qTAG-N-Blast-miniTurbo-LMNB1 | Donor template for Blast-2A-miniTurbo insertion into the N-terminus of the LMNB1 locus. To be co-transfected with sgRNA plasmid px330-PITCh-LMNB1 Addgene #207770 |
| 207775 | qTAG-N-Blast-ultraID-LMNB1 | Donor template for Blast-2A-ultraID insertion into the N-terminus of the LMNB1 locus. To be co-transfected with sgRNA plasmid px330-PITCh-LMNB1 Addgene #207770 |
| 207776 | qTAG-N-Blast-dTAG-LMNB1 | Donor template for Blast-2A-dTAG insertion into the N-terminus of the LMNB1 locus. To be co-transfected with sgRNA plasmid px330-PITCh-LMNB1 Addgene #207770 |
| 207777 | qTAG-N-Blast-3xFLAG-LMNB1 | Donor template for Blast-2A-3xFLAG insertion into the N-terminus of the LMNB1 locus. To be co-transfected with sgRNA plasmid px330-PITCh-LMNB1 Addgene #207770 |
| 207778 | qTAG-N-Blast-3xHA-LMNB1 | Donor template for Blast-2A-3xHA insertion into the N-terminus of the LMNB1 locus. To be co-transfected with sgRNA plasmid px330-PITCh-LMNB1 Addgene #207770 |
| 207779 | qTAG-N-Blast-V5-LMNB1 | Donor template for Blast-2A-V5 insertion into the N-terminus of the LMNB1 locus. To be co-transfected with sgRNA plasmid px330-PITCh-LMNB1 Addgene #207770 |
| 207780 | pX330-PITCh-H3C2 | Expresses SpCas9, the PITCh gRNA, and a sgRNA targeting the C-terminus of H3C2 for knock-in. |
| 207781 | qTAG-C-Solo-mScarlet-H3C2 | Donor template for mScarlet insertion into the C-terminus of the H3C2 locus for nuclei visualization. To be co-transfected with sgRNA plasmid px330-PITCh-H3C2 Addgene #207780 |
| 207782 | qTAG-C-mNeon-Blast-H3C2 | Donor template for mNeon-2A-Blast insertion into the C-terminus of the H3C2 locus for nuclei visualization. To be co-transfected with sgRNA plasmid px330-PITCh-H3C2 Addgene #207780 |
| 207783 | qTAG-C-moxGFP-Puro-H3C2 | Donor template for moxGFP-2A-Puro insertion into the C-terminus of the H3C2 locus for nuclei visualization. To be co-transfected with sgRNA plasmid px330-PITCh-H3C2 Addgene #207780 |
| 207784 | qTAG-C-miRFP670nano3-Blast-H3C2 | Donor template for miRFP670nano3-2A-Blast insertion into the C-terminus of the H3C2 locus for nuclei visualization. To be co-transfected with sgRNA plasmid px330-PITCh-H3C2 Addgene #207780 |
| 207785 | pX330-PITCh-TUBB4B | Expresses SpCas9, the PITCh gRNA, and a sgRNA targeting the C-terminus of TUBB4B for knock-in. |
| 207786 | qTAG-C-mNeon-Blast-TUBB4B | Donor template for mNeon-2A-Blast insertion into the C-terminus of the TUBB4B locus for tubulin visualization. To be co-transfected with sgRNA plasmid px330-PITCh-TUBB4B Addgene #207785 |
| 207788 | qTAG-C-moxGFP-Puro-LAMP1 | Donor template for moxGFP-2A-Puro insertion into the C-terminus of the LAMP1 locus. For lysosome visualization.To be co-transfected with sgRNA plasmid px330-LAMP1 (Addgene #207787) |
| 207790 | qTAG-C-mNeon-Puro-TOMM20 | Donor template for mNeon-2A-Puro insertion into the C-terminus of the TOMM20 locus. For mitochondria visualization. To be co-transfected with sgRNA plasmid px330-PITCh-TOMM20 (Addgene #207789) |
| 207791 | pX330-PITCh-GOLGA2 | Expresses SpCas9, the PITCh gRNA, and a sgRNA targeting the N-terminus of GOLGA2 for knock-in. |
| 207792 | qTAG-N-Puro-moxGFP-GOLGA2 | Donor template for Puro-2A-moxGFP insertion into the N-terminus of the GOLGA2 locus for Golgi visualization. To be co-transfected with sgRNA plasmid px330-PITCh-GOLGA2 Addgene #207791 |
| 207794 | qTAG-C-moxGFP-Puro-MAPRE1 | Donor template to insert moxGFP-2A-Puro into the C-terminus of the MAPRE1 locus for growing microtubule tip visualization. To be co-transfected with sgRNA plasmid px330-PITCh-MAPRE1 Addgene #207793 |