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Development of a V5-tag-directed nanobody and its implementation as an intracellular biosensor of GPCR signalling.

Zeghal M, Matte K, Venes A, Patel S, Laroche G, Sarvan S, Joshi M, Rain JC, Couture JF, Giguere PM
J Biol Chem. 2023 Jul 28:105107. doi: 10.1016/j.jbc.2023.105107. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
201473NbV5-eGFPIndirect microscopy/imaging of V5-tagged protein
201474NbV5-mCherryIndirect microscopy/imaging of V5-tagged protein
201475NbV5-LgBitNanoBit detection of V5-tagged protein
201476LgBit-NbV5NanoBit detection of V5-tagged protein
201477NbV5-GFP2BRET2 detection of V5-tagged protein
201478GFP2-NbV5BRET2 detection of V5-tagged protein
201479Rluc8-NbV5BRET detection of V5-tagged protein
201480NbV5-Rluc8BRET detection of V5-tagged protein
201481NbV5-TEV219TANGO detection of V5-tagged protein
201482NbV5-HALOTAGIndirect detection of V5-tagged protein using the HaloTag protein labeling technology.
201483NbAlfa-LgBitNanoBit detection of ALFA-tagged protein
201484LgBit-NbALFANanoBit detection of ALFA-tagged protein
201485NbAlfa-GFP2BRET2 detection of ALFA-tagged protein
201486GFP2-NbALFABRET2 detection of ALFA-tagged protein
201487NbALFA-TEV219TANGO detection of V5-tagged protein
201488NbV5-TEV219S153NTANGO detection of V5-tagged protein
201489NbV5-HiBiTNanoBit detection of V5-tagged protein
201490HiBiT-NbV5NanoBit detection of V5-tagged protein
201491V5-bArr1Expression of the beta-arrestin1 containing a N-terminal V5 tag.
201492bArr1-V5Expression of the beta-arrestin1 containing a C-terminal V5 tag.
201493V5-bArr2Expression of the beta-arrestin2 containing a N-terminal V5 tag.
201494bArr2-V5Expression of the beta-arrestin2 containing a N-terminal V5 tag.
201495ALFA-bArr1Expression of the beta-arrestin1 containing a N-terminal ALFA tag.
201496bArr1-ALFAExpression of the beta-arrestin1 containing a C-terminal ALFA tag.
201497ALFA-bArr2Expression of the beta-arrestin2 containing a N-terminal ALFA tag.
201498bArr2-ALFAExpression of the beta-arrestin2 containing a N-terminal ALFA tag.

Antibodies from Article