Skip to main content
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected]. Learn more


(Plasmid #24500)


Item Catalog # Description Quantity Price (USD)
Plasmid 24500 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.


  • Vector backbone
  • Backbone size w/o insert (bp) 4000
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin and Tetracycline, 100 & 10 μg/mL
  • Growth Temperature
  • Growth Strain(s)
  • Growth instructions
    Plasmid contains SupF. Grow in TOP10/P3 cells.
  • Copy number


  • Gene/Insert name
    Gq alpha
  • Alt name
    G protein alpha subunit
  • Alt name
  • Species
    M. musculus (mouse)
  • Mutation
    Gq alpha with the C-terminal amino acids changed from Gq alpha to Go alpha residues (EYNLV to GCGLY)
  • Entrez Gene
    Gnaq (a.k.a. 1110005L02Rik, 6230401I02Rik, Dsk1, Dsk10, Galphaq, Gq, GqI)
  • Tag / Fusion Protein
    • HA (see comments)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (unknown if destroyed)
  • 3′ cloning site NsiI (unknown if destroyed)
  • 5′ sequencing primer CMV-F
  • 3′ sequencing primer SP6
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

This is Gq alpha with the C-terminal amino acids changed from Gq alpha to Go alpha residues (EYNLV to GCGLY). Works the same as qi5 but (for unknown reasons) has a slightly lower basal PLC activity. This can increase the signal-to-noise ratio.

Contains an internal hemaglutinin epitope tag (DVPDYA) that has no effect on receptor coupling.

Please see supplemental document for G-protein chimera user manual.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    qo5 was a gift from Bruce Conklin (Addgene plasmid # 24500 ; ; RRID:Addgene_24500)
  • For your References section:

    Substitution of three amino acids switches receptor specificity of Gq alpha to that of Gi alpha. Conklin BR, Farfel Z, Lustig KD, Julius D, Bourne HR. Nature. 1993 May 20. 363(6426):274-6. 10.1038/363274a0 PubMed 8387644