qs5
(Plasmid #24498)

• Depositing Lab: Bruce Conklin
• Publication: Conklin et al Mol Pharmacol. 1996 Oct . 50(4):885-90.

Backbone

• Vector backbone: pcDNA1
• Backbone size w/o insert (bp): 4000
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin and Tetracycline, 100 & 10 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): Top10/P3
• Growth instructions: Plasmid contains SupF. Grow in TOP10/P3 cells.
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: Gq alpha
• Alt name: G protein alpha subunit
• Alt name: Gnaq
• Species: M. musculus (mouse)
• Mutation: Replacement of the five carboxyl-terminal amino acids of Gq alpha (EYNLV) with those of Gs alpha(QYELL)
• Entrez Gene: Gnaq (a.k.a. 1110005L02Rik, 6230401I02Rik, Dsk1, Dsk10, Galphaq, Gq, GqI)
• Tag / Fusion Protein:
  • HA (see comments)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRI (unknown if destroyed)
• 3′ cloning site: NsiI (unknown if destroyed)
• 5′ sequencing primer: CMV-F
• 3′ sequencing primer: SP6

Resource Information

• Supplemental Documents:
  • G-protein chimera user manual
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

This is Gq alpha with the C-terminal amino acids changed from Gq alpha to Gs alpha residues (EYNLV to QYELL). This construct allows some Gs-coupled receptors to stimulate phospholipase C.

Contains an internal hemaglutinin epitope tage (DVPDYA) that has no effect on receptor coupling.

Please see supplemental document for G-protein chimera user manual.