Full plasmid sequence is not available for this item.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||26725||Standard format: Plasmid sent in bacteria as agar stab||1||$85|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 4700
Vector typeMammalian Expression
Selectable markersNeomycin (select with G418)
Growth in Bacteria
Bacterial Resistance(s)Kanamycin, 50 μg/mL
Copy numberHigh Copy
SpeciesM. musculus (mouse)
MutationChanged Leucine 432 to Glycine to block ability of calpain to proteolyse talin
Entrez GeneTln1 (a.k.a. Tln)
/ Fusion Protein
- EGFP (N terminal on backbone)
- Cloning method Restriction Enzyme
- 5′ sequencing primer EGFP-C (Common Sequencing Primers)
Articles Citing this Plasmid
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
GFP-Talin L432G only differs from GFP-Talin WT in the codon comprised of base pairs 2677-2679. WT = CTT, L432G = GGT. This changes Leucine 432 to a Glycine
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:GFP-Talin-L432G was a gift from Anna Huttenlocher (Addgene plasmid # 26725 ; http://n2t.net/addgene:26725 ; RRID:Addgene_26725)
For your References section:Calpain-mediated proteolysis of talin regulates adhesion dynamics. Franco SJ, Rodgers MA, Perrin BJ, Han J, Bennin DA, Critchley DR, Huttenlocher A. Nat Cell Biol. 2004 Oct . 6(10):977-83. 10.1038/ncb1175 PubMed 15448700