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Depositing Lab
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Depositing OrganizationUniversity of Wisconsin, Madison
Located in United States of America -
Publication
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Citations
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
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| Plasmid | 32855 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
| DNA | 32855-D50 | 50 μg of DNA in Tris buffer | $495 | ||
| DNA | 32855-D100 | 100 μg of DNA in Tris buffer | $585 | ||
Backbone
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Vector backbonepEGFP-C1
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Backbone manufacturerBD Clontech
- Backbone size w/o insert (bp) 4749
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Modifications to backboneC1 vector frame shifted by cutting with BglII, blunting, and religating.
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Vector typeMammalian Expression
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameTalin 1 Rod aa 434-2541
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SpeciesM. musculus (mouse)
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Insert Size (bp)6327
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GenBank IDCAA39588.1
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Entrez GeneTln1 (a.k.a. Tln)
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Tag
/ Fusion Protein
- EGFP (N terminal on insert)
Resource Information
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A portion of this plasmid was derived from a plasmid made byEGFP-talin1 rod was cloned by removing the talin1 rod cDNA (encoding aa 434-2541) from the pJ6 Ω 7 vector (R. Hynes) and cloning it into the same frame-shifted pEGFP-C1 vector.
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please note that both the full plasmid sequence from the depositing laboratory and Addgene's sequencing results indicate a K673N mutation/SNP when the sequences are compared to GenBank entry NP_035732.2. The depositing laboratory states that this difference is not a concern for the function of the plasmid. The most appropriate reference sequence for the Talin1 insert in the plasmid is GenBank accession number CAA39588.1.
DNA (Catalog # 32855-D50)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 50 μg
- Concentration 1 μg/μL
- Pricing $495 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
DNA (Catalog # 32855-D100)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 100 μg
- Concentration 1 μg/μL
- Pricing $585 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
EGFP-talin1 rod was a gift from Anna Huttenlocher (Addgene plasmid # 32855 ; http://n2t.net/addgene:32855 ; RRID:Addgene_32855) -
For your References section:
Talin1 regulates TCR-mediated LFA-1 function. Simonson WT, Franco SJ, Huttenlocher A. J Immunol. 2006 Dec 1;177(11):7707-14. PubMed 17114441
Map uploaded by the depositor.
