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EGFP-talin1 rod
(Plasmid #32855)


Item Catalog # Description Quantity Price (USD)
Plasmid 32855 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.


  • Vector backbone
  • Backbone manufacturer
    BD Clontech
  • Backbone size w/o insert (bp) 4749
  • Modifications to backbone
    C1 vector frame shifted by cutting with BglII, blunting, and religating.
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
  • Growth Strain(s)
  • Copy number
    High Copy


  • Gene/Insert name
    Talin 1 Rod aa 434-2541
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
  • GenBank ID
  • Entrez Gene
    Tln1 (a.k.a. Tln)
  • Tag / Fusion Protein
    • EGFP (N terminal on insert)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    EGFP-talin1 rod was cloned by removing the talin1 rod cDNA (encoding aa 434-2541) from the pJ6 Ω 7 vector (R. Hynes) and cloning it into the same frame-shifted pEGFP-C1 vector.
  • Articles Citing this Plasmid

Terms and Licenses

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please note that both the full plasmid sequence from the depositing laboratory and Addgene's sequencing results indicate a K673N mutation/SNP when the sequences are compared to GenBank entry NP_035732.2. The depositing laboratory states that this difference is not a concern for the function of the plasmid. The most appropriate reference sequence for the Talin1 insert in the plasmid is GenBank accession number CAA39588.1.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    EGFP-talin1 rod was a gift from Anna Huttenlocher (Addgene plasmid # 32855 ; ; RRID:Addgene_32855)
  • For your References section:

    Talin1 regulates TCR-mediated LFA-1 function. Simonson WT, Franco SJ, Huttenlocher A. J Immunol. 2006 Dec 1;177(11):7707-14. PubMed 17114441