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D28 Cortactin GFP
(Plasmid #26723)

Full plasmid sequence is not available for this item.


This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 26723 Standard format: Plasmid sent in bacteria as agar stab 1 $85


  • Vector backbone
  • Backbone manufacturer
  • Backbone size w/o insert (bp) 4700
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
  • Growth Strain(s)
  • Copy number
    High Copy


  • Gene/Insert name
  • Alt name
    Cortical actin binding protein
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
  • Mutation
    Deletion removed 28 amino acids (aa# 334-361) encompassing the four calpain cleavage sites. All four sites are located immediately following the last half repeat of the actin binding domain and before the {alpha}-helical domain.
  • Entrez Gene
    Cttn (a.k.a. 1110020L01Rik, Ems1)
  • Tag / Fusion Protein
    • EGFP (N terminal on backbone)

Cloning Information

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    A FLAG-cortactin clone was a generous gift from A. Weaver (Vanderbilt University)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    D28 Cortactin GFP was a gift from Anna Huttenlocher (Addgene plasmid # 26723 ; ; RRID:Addgene_26723)
  • For your References section:

    Proteolysis of cortactin by calpain regulates membrane protrusion during cell migration. Perrin BJ, Amann KJ, Huttenlocher A. Mol Biol Cell. 2006 Jan . 17(1):239-50. 10.1091/mbc.e05-06-0488 PubMed 16280362