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Addgene

cyclin D1-HA-pBabe-Hygro
(Plasmid #22703)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 22703 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pBabe-Puro
  • Backbone size w/o insert (bp) 5536
  • Vector type
    Mammalian Expression, Retroviral
  • Selectable markers
    Hygromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    DH5alpha or XL1
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    cyclin D1
  • Alt name
    CCND1
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1159
  • Mutation
    wt cDNA
  • GenBank ID
    M64349
  • Entrez Gene
    CCND1 (a.k.a. BCL1, D11S287E, PRAD1, U21B31)
  • Tag / Fusion Protein
    • HA-tag (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamH I (not destroyed)
  • 3′ cloning site Sal I (not destroyed)
  • 5′ sequencing primer pBabe-5'
  • 3′ sequencing primer pBabe-3'
  • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Cyclin D1 cDNA insert from pBabePuro-CyclinD1 kindly provided by Dr. P. Sicinski, DFCI This insert is most probably derived from Addgene plasmid # 9050 (pBABE puro cyclinD1 HA ), deposited by William Hahn, DFCI

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    cyclin D1-HA-pBabe-Hygro was a gift from William Kaelin (Addgene plasmid # 22703 ; http://n2t.net/addgene:22703 ; RRID:Addgene_22703)
  • For your References section:

    Control of Cyclin D1 and Breast Tumorigenesis by the EglN2 Prolyl Hydroxylase. Zhang Q, Gu J, Li L, Liu J, Luo B, Cheung HW, Boehm JS, Ni M, Geisen C, Root DE, Polyak K, Brown M, Richardson AL, Hahn WC, Kaelin WG, Bommi-Reddy A. Cancer Cell. 2009 Nov 6. 16(5):413-24. 10.1016/j.ccr.2009.09.029 PubMed 19878873