166,806 results

1. pDP0437

   Plasmid

   #221127

   Purpose

   Plasmid for generating integration cassette to C-terminally tag gene of interest with pHluorin::HPHMX

   Depositor

   Allyson O’Donnell

   Article

   Oppenheimer et al Mol Biol Cell. 2024 Jul 1;35(7):mr5. doi

   Insert

   pHluorin
   

   Expression

   Yeast

   Available Since

   March 11, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pSec61β-mScarlet3-H (aka mYongHong)

   Plasmid

   #239414

   Purpose

   Expresses Sec61b-mScarlet3-H in mammalian cells

   Depositor

   Zhifei Fu

   Article

   Xiong et al Nat Methods. 2025 Apr 17. doi: 10.1038/s

   Insert

   Sec61β (SEC61B Human)
   

   Tags

   mScarlet3-H (aka mYongHong)

   Expression

   Mammalian

   Mutation

   M163H in mScarlet3

   Promoter

   CMV

   Available Since

   Aug. 4, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. 6xHis-SNAP-PI Probe

   Plasmid

   #211507

   Purpose

   For expression of recombinant biosensor for detection of Phosphatidylinositol (PI) from bacteria

   Depositor

   Hannes Maib, David Murray

   Article

   Maib et al J Cell Biol. 2024 Jun 3;223(6):e20231009

   Insert

   BcPI-PLC-ANH
   

   Tags

   6xhis-SNAP

   Expression

   Bacterial

   Available Since

   Jan. 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pPAP002

   Plasmid

   #153489

   Purpose

   Level 1 episomal expression plasmid for P. pastoris

   Depositor

   Sylvestre Marillonnet

   Article

   Pullmann et al Commun Biol. 2021 May 12;4(1):562. doi:

   Type

   Empty backbone

   Expression

   Yeast

   Available Since

   Jan. 28, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. 6xHsa.NFKB:d2EGFP

   Plasmid

   #239990

   Purpose

   Destabilized green fluorescent protein (d2EGFP) under transcriptional control of NF-kB activity

   Depositor

   Raquel Espin-Palazon

   Article

   Campbell et al Nat Commun. 2024 Sep 6;15(1):7787. doi:

   Insert

   Destabilized EGFP
   

   Promoter

   cfos minimal promoter

   Available Since

   June 27, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. pET-32a(+)-TGFB1

   Plasmid

   #120361

   Purpose

   Produce human recombinant TGFB1 in DE3 cells

   Depositor

   Paul Burridge

   Article

   Kuo et al Stem Cell Reports. 2020 Jan 9. pii: S221

   Insert

   Transforming Growth Factor-β1 (TGFB1 Human, Synthetic)
   

   Tags

   6x His and S-Tag

   Expression

   Bacterial

   Mutation

   Codon optimized for E. Coli production

   Promoter

   T7 Promoter

   Available Since

   June 28, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pET-11b_PcrA_M6_H93A

   Plasmid

   #194156

   Purpose

   Engineered PcrA_M6 helicase to support isothermal amplification method SHARP

   Depositor

   Taekjip Ha

   Article

   Gavrilov et al Nat Commun. 2022 Oct 23;13(1):6312. doi:

   Insert

   Engineered helicase PcrA_M6
   

   Tags

   6x His tag and FLAG tag

   Expression

   Bacterial

   Mutation

   H93A, C96A, N187C, C247A, L384V, L409C

   Available Since

   Jan. 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pXJ-Myc-IRF2

   Plasmid

   #232642

   Purpose

   transient expresses IRF2 in mammalian cells

   Depositor

   Chen-Yang Shen

   Article

   Chou et al NPJ Genom Med. 2022 Jan 19;7(1):4. doi:

   Insert

   IRF2 (IRF2 Human)
   

   Tags

   Myc

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   March 7, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. V96 ELP

   Plasmid

   #68392

   Purpose

   Elastin-like polypeptide (VPGxG) with 96 repeats of Valine guest residue

   Depositor

   Andrew MacKay

   Article

   Shah et al Protein Sci. 2012 Jun;21(6):743-50. doi:

   Insert

   V96 ELP
   

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Oct. 1, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. pD649-HAsp-PDCD1LG2-Fc(DAPA)-AviTag-6xHis

    Plasmid

    #156612

    Purpose

    Mammalian expression of cell-surface protein extracellular domain fused to Fc(DAPA)-Avi-6xHis. Protein is secreted from cells.

    Depositor

    Chris Garcia

    Article

    Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

    Insert

    PDCD1LG2 (PDCD1LG2 Human)
    

    Tags

    Fc(DAPA)-AviTag-6xHis

    Expression

    Mammalian

    Mutation

    Fc region of human IgG contains D265A and P329A m…

    Promoter

    CMV/SP6

    Available Since

    March 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCMV5 BRGI-Flag

    Plasmid

    #19143

    Depositor

    Joan Massague

    Article

    Xi et al J Biol Chem. 2008 Jan 11. 283(2):1146-55

    Insert

    SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a, member 4 (SMARCA4 Human)
    

    Tags

    Flag

    Expression

    Mammalian

    Available Since

    Sept. 3, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAAV-Syn-eGFP-2A-mCherry_D4H

    Plasmid

    #194875

    Purpose

    Ratiometric D4H sensor for in vivo neuron-specific cholesterol visualization

    Depositor

    Peter Scheiffele

    Article

    Czernecki et al bioRxiv 2022.11.16.516849

    Insert

    GFP-T2A-mCherry-D4H
    

    Use

    AAV

    Expression

    Mammalian

    Promoter

    human Synapsin

    Available Since

    Dec. 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pBABE puro human IRS-1

    Plasmid

    #11025

    Depositor

    Ronald Kahn

    Article

    Bruning et al Mol Cell Biol. 1997 Mar . 17(3):1513-21.

    Insert

    IRS-1 (IRS1 Human)
    

    Use

    Retroviral

    Expression

    Mammalian

    Available Since

    May 24, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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14. hrGFP-Keap1

    Plasmid

    #28025

    Depositor

    Qing Zhong

    Article

    Fan et al Autophagy. 2010 Jul 22. 6(5):.

    Insert

    Keap1 (KEAP1 Human)
    

    Tags

    hrGFP

    Expression

    Mammalian

    Available Since

    March 18, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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15. pDONR223-PDK4

    Plasmid

    #23577

    Depositor

    William Hahn, David Root

    Article

    Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

    Insert

    PDK4 (PDK4 Human)
    

    Use

    Gateway donor vector

    Available Since

    July 30, 2010

    Availability

    Academic Institutions and Nonprofits only

16. PB09-TRE-beta-globin-miR-ctrl-EF1a-GFP

    Plasmid

    #117319

    Purpose

    Overexpression of miR-124-surrounding locus (not miR-124) in eurkaryotic cells, encoded in a human beta-globin intron

    Depositor

    Volker Busskamp

    Article

    Kutsche et al Cell Syst. 2018 Sep 28. pii: S2405-4712(

    Insert

    hsa-miR-CTRL
    

    Use

    Transposon-mediated integration

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    miR-124 deleted

    Promoter

    Tet-inducible promoter

    Available Since

    Jan. 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pTR-471 IRF4-tNGFR

    Plasmid

    #186061

    Purpose

    Knockin of truncated NGFR to target gene

    Depositor

    Alexander Marson

    Article

    Shy et al Nat Biotechnol. 2022 Aug 25:10.1038/s415

    Insert

    IRF4 (IRF4 Human)
    

    Use

    CRISPR

    Mutation

    WT with tNGFR sequence

    Available Since

    July 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pZac2.1 gfaABC1D-cyto-GCaMP8f

    Plasmid

    #186050

    Purpose

    AAV-mediated expression of GCaMP8f under the control of GfaABC1D promoter

    Depositor

    Loren Looger

    Insert

    jGCaMP8f
    

    Use

    AAV

    Tags

    6xHis

    Expression

    Mammalian

    Promoter

    GfaABC1D

    Available Since

    Aug. 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pMT643

    Plasmid

    #8610

    Depositor

    Robert Hartley

    Article

    Hartley et al Biochemistry 1993 Jun 15;32(23):5978-84.

    Insert

    barstar A
    

    Expression

    Bacterial

    Mutation

    Two cysteines of barstar changed to alanine, C40A…

    Available Since

    Jan. 17, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCDH_blast_MCS_Nard_GFP_LAMIN

    Plasmid

    #167340

    Purpose

    Expresses GFP fused to lamin A (LA) in mammalian cells

    Depositor

    Tom Misteli

    Article

    Feric et al EMBO J. 2021 Mar 15;40(6):e107165. doi:

    Insert

    Lamin A (LMNA Human)
    

    Use

    Lentiviral

    Tags

    GFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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