|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||15713||Standard format: Plasmid sent in bacteria as agar stab||1||$85|
This material is available to academics and nonprofits only.
Vector backbonepBABE puro
Backbone manufacturerAvailable at Addgene (#1764)
- Backbone size w/o insert (bp) 5169
Vector typeMammalian Expression, Retroviral
Growth in Bacteria
Bacterial Resistance(s)Ampicillin, 100 μg/mL
Copy numberHigh Copy
Alt nameC/EBP beta isoform
SpeciesH. sapiens (human)
Insert Size (bp)500
Entrez GeneCEBPB (a.k.a. C/EBP-beta, IL6DBP, NF-IL6, TCF5)
- Cloning method Restriction Enzyme
- 5′ cloning site EcoRI (not destroyed)
- 3′ cloning site SalI (not destroyed)
- 5′ sequencing primer pBABE 5' (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
Human LIP was cloned by PCR from cDNA template and then digested with EcoR1/SalI and inserted in pBabe-Puro.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pBABE-puro LIP was a gift from Joan Massague (Addgene plasmid # 15713 ; http://n2t.net/addgene:15713 ; RRID:Addgene_15713)
For your References section:C/EBPbeta at the core of the TGFbeta cytostatic response and its evasion in metastatic breast cancer cells. Gomis RR, Alarcon C, Nadal C, Van Poznak C, Massague J. Cancer Cell. 2006 Sep . 10(3):203-14. 10.1016/j.ccr.2006.07.019 PubMed 16959612
Map uploaded by the depositor.