pCMV5 HA-TGFBRII
(Plasmid #19158)

• Depositing Lab: Joan Massague
• Publication: Joan Massague lab plasmids (unpublished)

Backbone

• Vector backbone: pCMV5
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Transforming growth factor, beta receptor II
• Alt name: AAT3; FAA3; MFS2; RIIC; LDS1B; LDS2B; TAAD2; TGFR-2; TGFbeta-RII
• Species: H. sapiens (human)
• Mutation: PCR based mutagenesis
• Entrez Gene: TGFBR2 (a.k.a. AAT3, FAA3, LDS1B, LDS2, LDS2B, MFS2, RIIC, TAAD2, TBR-ii, TBRII, TGFR-2, TGFbeta-RII, tbetaR-II)
• Tag / Fusion Protein:
  • HA (YDVPDYASL) (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: SalI (destroyed during cloning)
• 3′ cloning site: BamHI (not destroyed)
• 5′ sequencing primer: CMV Forward

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Jacqueline Doody
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

See author map for cloning details.