Full plasmid sequence is not available for this item.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||34700||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 4900
Vector typeBacterial Expression
Growth in Bacteria
Copy numberHigh Copy
SpeciesH. sapiens (human)
- Promoter tac
/ Fusion Protein
- GST (N terminal on backbone)
- Cloning method Restriction Enzyme
- 5′ cloning site BamHI (not destroyed)
- 3′ cloning site EcoRI (not destroyed)
- 5′ sequencing primer pGEX 3' (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:GST-PRMT6 was a gift from Steven Clarke (Addgene plasmid # 34700 ; http://n2t.net/addgene:34700 ; RRID:Addgene_34700)
For your References section:The novel human protein arginine N-methyltransferase PRMT6 is a nuclear enzyme displaying unique substrate specificity. Frankel A, Yadav N, Lee J, Branscombe TL, Clarke S, Bedford MT. J Biol Chem. 2002 Feb 1;277(5):3537-43. Epub 2001 Nov 27. 10.1074/jbc.M108786200 PubMed 11724789