166,778 results

1. pHyo094 (eMutaT7)

   Plasmid

   #173124

   Purpose

   Expresses eMutaT7 for mutagenizing genes downstream from a T7 promoter in e.coli

   Depositor

   Seokhee Kim

   Article

   Park et al Nucleic Acids Res. 2021 Apr 6;49(6):e32.

   Insert

   pmCDA1-T7RNAP
   

   Expression

   Bacterial

   Available Since

   Sept. 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pHLmMBP-8

   Plasmid

   #72347

   Purpose

   Mammalian expression of cytoplasmic mMBP TEV cleavage site-linked fusion proteins with C-terminal 6His-tag/Strep-tag II/HA-tag

   Depositor

   Luca Jovine

   Article

   Bokhove et al J Struct Biol. 2016 Feb 3. 194:1-7. pii

   Type

   Empty backbone

   Tags

   6His-tag, HA-tag, Strep-tag II, and mMBP with C-t…

   Expression

   Mammalian

   Promoter

   CMV enhancer + chicken beta-actin promoter

   Available Since

   Feb. 17, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCMV-OptoTGFBRs

   Plasmid

   #118942

   Purpose

   Mammalian expression plasmid of OptoTGFBRs (optogenetically-activated TGFb receptors)

   Depositor

   Won Do Heo

   Article

   Li et al ACS Synth Biol. 2018 Feb 16;7(2):443-451

   Insert

   OptoTGFBRs (Tgfb1, Tgfbr2 Mustard Weed, Mouse)
   

   Tags

   tdTomato

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   June 26, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pMIA4.721-Olônne

   Plasmid

   #214443

   Purpose

   BxbI landing-pad HDR donor for targeting Olônne-18 GSH. CAGG-driven Casp9-P2A-Bsd.

   Depositor

   Roger Foo

   Article

   Autio et al Elife. 2024 Jan 2;13:e79592. doi: 10.755

   Insert

   2xFKBP12-Casp9
   

   Use

   Hdr donor

   Tags

   P2A-Bsd

   Available Since

   April 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. LT3GEPIR-CDK1shRNA1

   Plasmid

   #220569

   Purpose

   To inducibly knockdown CDK1 expression

   Depositor

   Sean Lee

   Insert

   CDK1 shRNA1 (CDK1 Human)
   

   Use

   Lentiviral

   Promoter

   TRE3G (TetOP) promoter

   Available Since

   July 3, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. TRE reporter backbone

   Plasmid

   #158678

   Purpose

   This plasmid serves as the backbone for the TRE reporters described in our paper. It's a promoterless-mStrawberry-pGK-BSD backbone to which we insert a pathway specific promoter before the mStrawberry

   Depositor

   Ravid Straussman

   Article

   Kaufman et al Nat Commun. 2022 May 18;13(1):2725. doi:

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   mStrawberry

   Expression

   Mammalian

   Available Since

   May 18, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. TFORF2029

   Plasmid

   #143566

   Purpose

   Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

   Depositor

   Feng Zhang

   Article

   Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

   Insert

   SMARCA5 (SMARCA5 Human)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   EF1a

   Available Since

   Feb. 27, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. TFORF1326

   Plasmid

   #141811

   Purpose

   Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

   Depositor

   Feng Zhang

   Article

   Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

   Insert

   KLF15 (KLF15 Human)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   EF1a

   Available Since

   Jan. 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCMVβ-p300.DY-myc

   Plasmid

   #30490

   Depositor

   Tso-Pang Yao

   Insert

   p300 (EP300 Human)
   

   Tags

   Myc

   Expression

   Mammalian

   Mutation

   aspartic acid 1399 converted to tyrosine (Acetyla…

   Available Since

   Oct. 6, 2011

   Availability

   Academic Institutions and Nonprofits only

10. pCLX-PGK-hCDK4

    Plasmid

    #114314

    Purpose

    Constitutive expression of the CDK4 protein

    Depositor

    Patrick Salmon

    Insert

    CDK4 (CDK4 Human)
    

    Use

    Lentiviral

    Available Since

    Sept. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. TFORF2987

    Plasmid

    #144463

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    RORA (RORA Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    Sept. 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pFastBac1-Ac-AChBP

    Plasmid

    #63128

    Purpose

    Expression of Aplysia californica AChBP

    Depositor

    Titia Sixma

    Article

    Celie et al Nat Struct Mol Biol. 2005 Jul;12(7):582-

    Insert

    AChBP
    

    Expression

    Insect

    Promoter

    Polyhedrin

    Available Since

    March 13, 2015

    Availability

    Academic Institutions and Nonprofits only

13. 6xHis-SNAP-PI Probe

    Plasmid

    #211507

    Purpose

    For expression of recombinant biosensor for detection of Phosphatidylinositol (PI) from bacteria

    Depositor

    Hannes Maib, David Murray

    Article

    Maib et al J Cell Biol. 2024 Jun 3;223(6):e20231009

    Insert

    BcPI-PLC-ANH
    

    Tags

    6xhis-SNAP

    Expression

    Bacterial

    Available Since

    Jan. 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcDNA3-PM-ExRai-AktAR2

    Plasmid

    #184050

    Purpose

    Plasma membrane-targeted ExRai-AktAR2

    Depositor

    Jin Zhang

    Article

    Chen et al ACS Cent Sci. 2021 Dec 22;7(12):2009-202

    Insert

    PM-ExRai-AktAR2
    

    Tags

    N-terminal targeting sequence from Lyn kinase

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    June 2, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pN3-SP4FL

    Plasmid

    #107719

    Purpose

    Expression plasmid for full-length human Sp4

    Depositor

    Guntram Suske

    Insert

    Sp4 (SP4 Human)
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 18, 2018

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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16. pUAS-PE2-attB

    Plasmid

    #149550

    Purpose

    Expression of PE2 enzyme under control of the Gal4-regulated UAS promoter. Can be used to generate transgenic flies with white+ selection.

    Depositor

    Norbert Perrimon

    Article

    Bosch et al Proc Natl Acad Sci U S A. 2021 Jan 5;118

    Insert

    PE2
    

    Use

    CRISPR

    Expression

    Insect

    Promoter

    UAS

    Available Since

    Aug. 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pMXs-IP-PEX3-EGFP

    Plasmid

    #228142

    Purpose

    Expresses PEX3-GFP in mammalian cells

    Depositor

    Noboru Mizushima

    Article

    Kurikawa et al PLoS Biol. 2024 Sep 17;22(9):e3002777. d

    Insert

    PEX3 (PEX3 Human)
    

    Use

    Retroviral

    Tags

    EGFP

    Expression

    Mammalian

    Available Since

    Nov. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pMZ827

    Plasmid

    #155321

    Purpose

    Constitutive expression of E-PIF6(1-100) in plants

    Depositor

    Matias Zurbriggen

    Article

    Ochoa-Fernandez et al Nat Methods. 2020 Jul;17(7):717-725. doi

    Insert

    PCaMV35S-E-PIF6(1-100)-NLS-Tnos
    

    Use

    Synthetic Biology

    Expression

    Plant

    Available Since

    Oct. 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pROF051

    Plasmid

    #155328

    Purpose

    Constitutive expression of SRDX-EL222 in plants

    Depositor

    Matias Zurbriggen

    Article

    Ochoa-Fernandez et al Nat Methods. 2020 Jul;17(7):717-725. doi

    Insert

    PCaMV35S-SRDX-NLS-EL222-Tnos
    

    Use

    Synthetic Biology

    Expression

    Plant

    Available Since

    Oct. 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pcDNA3-EGFP-RhoA-wt

    Plasmid

    #12965

    Depositor

    Gary Bokoch

    Article

    Subauste et al J Biol Chem. 2000 Mar 31. 275(13):9725-3

    Insert

    RhoA (RHOA Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Available Since

    Oct. 27, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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