162,700 results

1. pAAV-pMecp2-dSaCas9-KRAB-pU6-sgRNA

   Plasmid

   #158988

   Purpose

   Vector E encodes pAAV-pMecp2-dSaCas9-KRAB-spA-pU6-sgRNA (BsaI) transgenes for AAV packaging and expression of CRISPR interference in neurons

   Depositor

   Chung Tin

   Article

   Lau et al Mol Ther Nucleic Acids. 2019 Jun 7;16:63

   Insert

   dSaCas9-KRAB
   

   Use

   AAV and CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   pMecp2

   Available since

   Aug. 29, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. CAHS2 (pBS0249)

   Plasmid

   #185139

   Purpose

   For the mammalian expression of the tardigrade protein CAHS2. This is one of the bottom performing targets in our apoptosis assay (it induced apoptosis).

   Depositor

   Pamela Silver

   Article

   Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

   Insert

   CAHS2
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 5, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCMV-PE2-P2A-hMLH1dn

   Plasmid

   #174827

   Purpose

   Mammalian expression of SpCas9 prime editor 2 with P2A-human MLH1dn (codon optimized)

   Depositor

   David Liu

   Article

   Chen et al Cell. 2021 Oct 28;184(22):5635-5652.e29.

   Insert

   PE2-P2A-hMLH1dn
   

   Use

   Tags

   SV40 bpNLS

   Expression

   Mammalian

   Mutation

   Detailed in manuscript; deletion of residues 754-…

   Promoter

   CMV

   Available since

   Oct. 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. AAV-CAG-Cre

   Plasmid

   #196140

   Purpose

   AAV-mediated expression of Cre under the CAG promoter .

   Depositor

   Edward Boyden

   Article

   Chuong et al Nat Neurosci. 2014 Aug;17(8):1123-9. doi

   Insert

   Cre
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   codon diversified

   Promoter

   CAG

   Available since

   June 30, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA-FLAG-REL

   Plasmid

   #27253

   Depositor

   Thomas Gilmore

   Article

   Leeman et al Oncogene. 2008 Dec 4. 27(53):6770-81.

   Insert

   REL (REL Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 1, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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6. HA-GABARAPL2

   Plasmid

   #137761

   Purpose

   Expression of GABARAPL2

   Depositor

   Carol Mercer

   Article

   Stefely et al Autophagy. 2020 Jan 23. doi: 10.1080/155

   Insert

   GABARAPL2 (GABARAPL2 Human)
   

   Use

   Tags

   HA and myc

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 27, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pPB-US-ECasE

   Plasmid

   #83961

   Purpose

   Piggyac transposon containing tamoxifen inducible Cas9 and a gRNA cassette.

   Depositor

   Eleanor Chen

   Insert

   ERT2-spCas9-ERT2
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   please see depositor comments below

   Promoter

   U6 (gRNA), EF1a (ERT2-Cas9-ERT2)

   Available since

   Dec. 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAC1802_pmax-RBFOX1N-dCasRx-C

   Plasmid

   #118635

   Purpose

   Transient transfection; Expresses RBFOX1N-dCasRx-C; CAGGS promoter

   Depositor

   Albert Cheng

   Article

   Du et al Nat Commun. 2020 Jun 12;11(1):2973. doi:

   Insert

   RBFOX1N-dCasRx-C
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAGGS

   Available since

   Jan. 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. PKC alpha WT

   Plasmid

   #21232

   Depositor

   Bernard Weinstein

   Article

   Soh et al J Biol Chem. 2003 Sep 5. 278(36):34709-1

   Insert

   PKC alpha (PRKCA Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 13, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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10. MSCV JMJD3

    Plasmid

    #21212

    Depositor

    Paul Khavari

    Article

    Sen et al Genes Dev. 2008 Jul 15. 22(14):1865-70.

    Insert

    JMJD3 (KDM6B Human)
    

    Use

    Retroviral

    Tags

    Flag and HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 22, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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11. CS2 Flag-Smad2

    Plasmid

    #14042

    Depositor

    Joan Massague

    Article

    Hata et al Nature. 1997 Jul 3. 388(6637):82-7.

    Insert

    Smad2 (SMAD2 Human)
    

    Use

    Xenopus/mammalian/avian/zebrafish

    Tags

    Flag

    Expression

    Mutation

    Promoter

    Available since

    May 10, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLX313 MSLN

    Plasmid

    #193617

    Purpose

    Expresses MSLN

    Depositor

    William Hahn

    Article

    Hagel et al Cancer Res. 2022 Dec 22:CAN-22-2245. doi

    Insert

    MSLN (MSLN Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pMXs-mPKR-FLAG-IG

    Plasmid

    #101792

    Purpose

    Retroviral vector encoding C-terminally FLAG tagged mouse PKR

    Depositor

    Seiichi Oyadomari

    Article

    Taniuchi et al Sci Rep. 2016 Sep 16;6:32886. doi: 10.10

    Insert

    Pkr (Eif2ak2 Mouse)
    

    Use

    Retroviral

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    LTR

    Available since

    Oct. 2, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLenti-hSyn1-mRuby2-T2A-GCaMP6f

    Plasmid

    #197595

    Purpose

    Lentiviral transfer vector for the neuronal expression of mRuby2-T2A-GCaMP6f (fluorescent reporter for Calcium imaging)

    Depositor

    Patrik Verstreken

    Insert

    mRuby2-T2A-GCaMP6f
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    hSynI

    Available since

    April 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCMVHA hEZH2

    Plasmid

    #24230

    Purpose

    Mammalian expression of human EZH2 with HA tag

    Depositor

    Kristian Helin

    Article

    Bracken et al EMBO J. 2003 Oct 15. 22(20):5323-35.

    Insert

    EZH2 (EZH2 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 25, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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16. mEmerald-N1

    Plasmid

    #53976

    Purpose

    Localization: N1 Cloning Vector, Excitation: 487, Emission: 509

    Depositor

    Michael Davidson

    Type

    Empty backbone

    Use

    Tags

    mEmerald

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 16, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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17. pSL1423

    Plasmid

    #194904

    Purpose

    pSLI-TGD/TurboID, C-terminal tag of recodonized TurboID for Plasmodium falciparum

    Depositor

    Scott Lindner

    Insert

    TurboID
    

    Use

    Plasmodium falciparum

    Tags

    GFPmut2

    Expression

    Mutation

    Promoter

    None. This allows a C-terminal fusion to a protei…

    Available since

    Feb. 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pDONR223_PIK3CD_WT

    Plasmid

    #82222

    Purpose

    Gateway Donor vector containing PIK3CD , part of the Target Accelerator Plasmid Collection.

    Depositor

    David Root, William Hahn, Jesse Boehm

    Article

    Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

    Insert

    PIK3CD (PIK3CD Human)
    

    Use

    Gateway entry vector

    Tags

    Expression

    Mutation

    WT

    Promoter

    None

    Available since

    April 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. pRSFDuet-1_VNp-mNeongreen-Etanercept

    Plasmid

    #182421

    Purpose

    Bacterial expression of Vesicle Nucleating peptide-mNeongreen-Etanercept fusion

    Depositor

    Dan Mulvihill

    Article

    Eastwood et al Cell Rep Methods. 2023 Feb 2;3(2):100396

    Insert

    VNp-mNeongreen-Etanercept (TNFRSF1B Human)
    

    Use

    Tags

    Vesicle Nucleating peptide (VNp) & mNeongreen

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Feb. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pcDNA-iLACCO1.2

    Plasmid

    #218823

    Purpose

    Mammalian expression of genetically encoded green fluorescent L-lactate biosensor with high affinity

    Depositor

    Robert Campbell

    Article

    Hario et al ACS Cent Sci. 2024 Jan 31;10(2):402-416.

    Insert

    iLACCO1.2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 30, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge