Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

In Category

Filter By

161,152 results

Showing: 10581 - 10600 of 161152 results

  1. pTriEx-mCherry-PA-Rac1-C450A

    Plasmid
    #22028
    Purpose
    Expression of Rac1 fused with light-insensitive LOV2 (C450A mutation)
    Depositor
    Klaus Hahn
    Article
    Wu et al Nature. 2009 Sep 3. 461(7260):104-8.
    Insert
    PA-Rac1-C450A (RAC1 Avena sativa (oat), Human)
    Use
    Tags
    6xHis and mCherry
    Expression
    Bacterial, Insect, and Mamm…
    Mutation
    LOV(Leu404-Leu546, C450A). Rac1 starts at Ile4 an…
    Promoter
    CMV, p10
    Available since
    Sept. 29, 2009
    Availability
    Academic Institutions and Nonprofits only
    22028-plasmid-map-sequence-id-213358

    Enlarge

  5. pSB1A3 EcoRI-RTX with EcoRI Methylase-AmilCP

    Plasmid
    #85165
    Purpose
    Provides the coding region to express the EcoRI restriction enzyme. The RTX tag allows the EcoRI enzyme to be isolated via calcium-precipitation.
    Depositor
    Robin Dowell
    Article
    Hendrix et al ACS Synth Biol. 2014 Dec 19;3(12):969-71
    Insert
    EcoRI-RTX and EcoRI Methylase-AmilCP coding sequences
    Use
    Tags
    AmilCP and RTX
    Expression
    Bacterial
    Mutation
    Promoter
    J23100 promoter
    Available since
    Dec. 20, 2016
    Availability
    Academic Institutions and Nonprofits only
    85165-plasmid-map-sequence-id-354765

    Enlarge

  7. GFP-ESR1

    Plasmid
    #65211
    Purpose
    Full length mammalian expression vector for hESR1
    Depositor
    Ratna Vadlamudi
    Insert
    esr1 (ESR1 Human)
    Use
    Tags
    AcGFP
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    June 29, 2015
    Availability
    Academic Institutions and Nonprofits only
    65211-plasmid-map-sequence-id-422716

    Enlarge

  9. pJ3 SHP1 WT

    Plasmid
    #8572
    Depositor
    Ben Neel
    Insert
    SHP1 (PTPN6 Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    June 17, 2005
    Availability
    Academic Institutions and Nonprofits only

  10. RABGEF1A-c014

    Plasmid
    #175478
    Purpose
    Protein expression in insect cells. Entire CDS, M1-G491. Codon optimised.
    Depositor
    Opher Gileadi
    Insert
    RABGEF1 (RABGEF1 Human)
    Use
    Tags
    His6-Zbasic-TEV
    Expression
    Insect
    Mutation
    Promoter
    Available since
    Jan. 27, 2022
    Availability
    Academic Institutions and Nonprofits only
    175478-plasmid-map-sequence-id-353251

    Enlarge

  11. MIP 339 CoMiP coLin28 2A Nanog IRES eGFP

    Plasmid
    #63729
    Purpose
    To express codon optimized Lin-28 and wild type Nanog. A single plasmid reprogramming system using a mini-intronic plasmid (MIP).
    Depositor
    Mark Kay, Joseph Wu
    Article
    Diecke et al Sci Rep. 2015 Jan 28;5:8081. doi: 10.103
    Inserts
    Lin28 (LIN28A Human)
    Nanog (NANOG Human)
    Use
    Tags
    T2A
    Expression
    Mammalian
    Mutation
    codon optimized
    Promoter
    SFFV
    Available since
    June 1, 2015
    Availability
    Academic Institutions and Nonprofits only
    63729-plasmid-map-sequence-id-429278

    Enlarge

  18. pSpdCas9-huTET1CD-T2A-GFP (PX458)

    Plasmid
    #129025
    Purpose
    targeted DNA demethylation, expression of dCas9-huTET1CD-T2A-EGFP and cloning backbone for sgRNA
    Depositor
    Julia K Polansky
    Article
    Kressler et al Front Immunol. 2021 Jan 7;11:609891. doi
    Insert
    dCas9-huTET1CD, SgRNA cloning site
    Use
    Tags
    HA-Tag, NLS and T2A-EGFP
    Expression
    Mammalian
    Mutation
    dCas9 (D10A;H840A)
    Promoter
    CbH (for dCas9-huTET1CD-T2A-EGFP) U6 (for sgRNA)
    Available since
    Jan. 25, 2021
    Availability
    Academic Institutions and Nonprofits only
    129025-plasmid-map-sequence-id-454365

    Enlarge

  19. pLV-H1-SGINX

    Plasmid
    #174844
    Purpose
    empty vector for cloning shRNA and/or miRNA
    Depositor
    Helen Morrison
    Type
    Empty backbone
    Use
    Lentiviral and RNAi
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Oct. 8, 2021
    Availability
    Academic Institutions and Nonprofits only
    174844-plasmid-map-sequence-id-344689

    Enlarge

Showing: 10581 - 10600 of 161152 results