pMGIB-3xFLAG-dyskerin
(Plasmid #53607)

• Purpose: Retroviral expression of human dyskerin
• Depositing Lab: Steven Artandi
• Publication: Venteicher et al Science. 2009 Jan 30;323(5914):644-8. doi: 10.1126/science.1165357.

Backbone

• Vector backbone: pMGIB
• Backbone manufacturer: -
• Backbone size w/o insert (bp): 6000
• Total vector size (bp): 7000
• Modifications to backbone: Retroviral backbone. Insert followed by IRES-Blasticidin cassette
• Vector type: Mammalian Expression, Retroviral
• Selectable markers: Blasticidin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: dyskerin
• Alt name: DKC1
• Species: H. sapiens (human)
• GenBank ID: isoform 1
• Entrez Gene: DKC1 (a.k.a. CBF5, CHINE1, DKC, DKCX, NAP57, NOLA4, XAP101)
• Promoter: LTR
• Tag / Fusion Protein:
  • 3xFLAG (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: AscI (not destroyed)
• 3′ cloning site: PacI (not destroyed)
• 5′ sequencing primer: T3
• 3′ sequencing primer: IRES-R

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry