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pCDH-3xFLAG-TERT
(Plasmid #51631)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 51631 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pCDH
  • Backbone manufacturer
    unknown
  • Modifications to backbone
    N terminal 3xFLAG tag after Kozak
  • Vector type
    Mammalian Expression, Lentiviral
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    TERT
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    3399
  • GenBank ID
  • Entrez Gene
    TERT (a.k.a. CMM9, DKCA2, DKCB4, EST2, PFBMFT1, TCS1, TP2, TRT, hEST2, hTRT)
  • Tag / Fusion Protein
    • 3xFLAG (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site AscI (not destroyed)
  • 3′ cloning site PacI (not destroyed)
  • 5′ sequencing primer CMV
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCDH-3xFLAG-TERT was a gift from Steven Artandi (Addgene plasmid # 51631 ; http://n2t.net/addgene:51631 ; RRID:Addgene_51631)
  • For your References section:

    TPP1 OB-fold domain controls telomere maintenance by recruiting telomerase to chromosome ends. Zhong FL, Batista LF, Freund A, Pech MF, Venteicher AS, Artandi SE. Cell. 2012 Aug 3;150(3):481-94. doi: 10.1016/j.cell.2012.07.012. 10.1016/j.cell.2012.07.012 PubMed 22863003