pCDNA-3xFLAG-TCAB1
(Plasmid #51630)

• Purpose: Mammalian expression of 3xFLAG tagged TCAB1, N-terminal.
• Depositing Lab: Steven Artandi
• Publication: Venteicher et al Science. 2009 Jan 30;323(5914):644-8. doi: 10.1126/science.1165357.

Backbone

• Vector backbone: pcDNA3.1
• Backbone manufacturer: Invitrogen/LIfe Technologies
• Backbone size w/o insert (bp): 5500
• Modifications to backbone: N terminal 3xFLAG tag after Kozak
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: TCAB1
• Alt name: WRAP53
• Species: H. sapiens (human)
• Insert Size (bp): 1647
• GenBank ID: NM_001143990
• Entrez Gene: WRAP53 (a.k.a. DKCB3, TCAB1, WDR79)
• Promoter: CMV
• Tag / Fusion Protein:
  • 3xFLAG (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: AscI (not destroyed)
• 3′ cloning site: PacI (not destroyed)
• 5′ sequencing primer: pCMV promoter forward
• 3′ sequencing primer: bGH reverse

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry