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Addgene

pCWXPGR-pTF-betaCatenin
(Plasmid #114281)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 114281 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pCWXPGR65
  • Vector type
    Lentiviral
  • Selectable markers
    GFP

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Catenin beta 1
  • Species
    H. sapiens (human)
  • Mutation
    deletion of AA 1 to 45
  • Entrez Gene
    CTNNB1 (a.k.a. CTNNB, EVR7, MRD19, NEDSDV, armadillo)
  • Tag / Fusion Protein
    • FLAG (N terminal on insert)

Cloning Information

  • Cloning method Gateway Cloning

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

pTF is an optimized TET-promoter, described in HGT 2011, Giry et al.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCWXPGR-pTF-betaCatenin was a gift from Patrick Salmon (Addgene plasmid # 114281 ; http://n2t.net/addgene:114281 ; RRID:Addgene_114281)
  • For your References section:

    Wnt signaling regulates multipolar-to-bipolar transition of migrating neurons in the cerebral cortex. Boitard M, Bocchi R, Egervari K, Petrenko V, Viale B, Gremaud S, Zgraggen E, Salmon P, Kiss JZ. Cell Rep. 2015 Mar 3;10(8):1349-61. doi: 10.1016/j.celrep.2015.01.061. Epub 2015 Feb 26. 10.1016/j.celrep.2015.01.061 PubMed 25732825