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pXL004-ishRNA-scramble
(Plasmid #36311)

Full plasmid sequence is not available for this item.

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 36311 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pLV-H1TetO-RFP-Puro
  • Backbone manufacturer
    Biosettia
  • Backbone size w/o insert (bp) 10275
  • Vector type
    Mammalian Expression, Lentiviral, RNAi
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Stbl3
  • Growth instructions
    18 hrs at 250 rpm
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    shRNA of scramble
  • Alt name
    scramble
  • gRNA/shRNA sequence
    GCTACACTATCGAGCAATT
  • Insert Size (bp)
    1008
  • Promoter TetO-H1
  • Tag / Fusion Protein
    • EF1a-TetR-IRES-RFP-Puro (C terminal on backbone)

Cloning Information

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

The plasmid backbone is TetO-H1-shRNA-EF1a-TetR-IRES-RFP-Puro. It is a all-in-one inducible shRNA knockdown lentiviral plasmid.

The paper associated with this plasmid is "Robust cardiomyocyte differentiation from human pluripotent stem cells via temporal modulation of canonical Wnt signaling"

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pXL004-ishRNA-scramble was a gift from Sean Palecek (Addgene plasmid # 36311 ; http://n2t.net/addgene:36311 ; RRID:Addgene_36311)
  • For your References section:

    Robust cardiomyocyte differentiation from human pluripotent stem cells via temporal modulation of canonical Wnt signaling. Lian X, Hsiao C, Wilson G, Zhu K, Hazeltine LB, Azarin SM, Raval KK, Zhang J, Kamp TJ, Palecek SP. Proc Natl Acad Sci U S A. 2012 May 29. 10.1073/pnas.1200250109 PubMed 22645348