pLV-beta-catenin deltaN90
(Plasmid #36985)

• Depositing Lab: Bob Weinberg
• Publication: Guo et al Cell. 2012 Mar 2;148(5):1015-28.

Backbone

• Vector backbone: pLV-IRES-neo
• Backbone manufacturer: I. Ben-Porath
• Modifications to backbone: pLV-IRES-neo (a gift from I. Ben-Porath) was generated by replacing dsRed2 in pLV-tTRKRAB-Red (Addgene plasmid 12250) with the neomycin-resistance gene and removing the sequence of tTR-KRAB.
• Vector type: Mammalian Expression, Lentiviral
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): Stbl3
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: beta-catenin deltaN90
• Alt name: beta-catenin
• Alt name: CTNNB1
• Species: H. sapiens (human)
• Mutation: Deletion of amino acids 1-90 (Constitutively Active); S374G in beta-catenin
• Entrez Gene: CTNNB1 (a.k.a. CTNNB, EVR7, MRD19, NEDSDV, armadillo)
• Promoter: EF1a
• Tag / Fusion Protein:
  • Myc (N terminal on insert)

Cloning Information

• Cloning method: Unknown
• 5′ sequencing primer: EF1a-fwd
• 3′ sequencing primer: WPRE-R

Resource Information

• Supplemental Documents:
  • pLV-tTRKRAB-Red vector map
• Articles Citing this Plasmid:
  • 5 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

The pLV-neo vector was modified from the attached vector by replacing dsRed with Neomycin resistant cassette and removing tTR-kRAB.

Addgene QC sequence analysis identified a S374G discrepancy, relative to reference sequence NP_001091679.1.