|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||36985||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Backbone manufacturerI. Ben-Porath
Modifications to backbonepLV-IRES-neo (a gift from I. Ben-Porath) was generated by replacing dsRed2 in pLV-tTRKRAB-Red (Addgene plasmid 12250) with the neomycin-resistance gene and removing the sequence of tTR-KRAB.
Vector typeMammalian Expression, Lentiviral
Selectable markersNeomycin (select with G418)
Growth in Bacteria
Gene/Insert namebeta-catenin deltaN90
SpeciesH. sapiens (human)
MutationDeletion of amino acids 1-90 (Constitutively Active); S374G in beta-catenin
Entrez GeneCTNNB1 (a.k.a. CTNNB, EVR7, MRD19, NEDSDV, armadillo)
- Promoter EF1a
/ Fusion Protein
- Myc (N terminal on insert)
- Cloning method Unknown
- 5′ sequencing primer EF1a-fwd
- 3′ sequencing primer WPRE-R (Common Sequencing Primers)
The pLV-neo vector was modified from the attached vector by replacing dsRed with Neomycin resistant cassette and removing tTR-kRAB.
Addgene QC sequence analysis identified a S374G discrepancy, relative to reference sequence NP_001091679.1.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pLV-beta-catenin deltaN90 was a gift from Bob Weinberg (Addgene plasmid # 36985 ; http://n2t.net/addgene:36985 ; RRID:Addgene_36985)
For your References section:Slug and Sox9 cooperatively determine the mammary stem cell state. Guo W, Keckesova Z, Donaher JL, Shibue T, Tischler V, Reinhardt F, Itzkovitz S, Noske A, Zurrer-Hardi U, Bell G, Tam WL, Mani SA, van Oudenaarden A, Weinberg RA. Cell. 2012 Mar 2;148(5):1015-28. 10.1016/j.cell.2012.02.008 PubMed 22385965