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pLV-beta-catenin deltaN90
(Plasmid #36985)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 36985 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pLV-IRES-neo
  • Backbone manufacturer
    I. Ben-Porath
  • Modifications to backbone
    pLV-IRES-neo (a gift from I. Ben-Porath) was generated by replacing dsRed2 in pLV-tTRKRAB-Red (Addgene plasmid 12250) with the neomycin-resistance gene and removing the sequence of tTR-KRAB.
  • Vector type
    Mammalian Expression, Lentiviral
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Stbl3
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    beta-catenin deltaN90
  • Alt name
    beta-catenin
  • Alt name
    CTNNB1
  • Species
    H. sapiens (human)
  • Mutation
    Deletion of amino acids 1-90 (Constitutively Active); S374G in beta-catenin
  • Entrez Gene
    CTNNB1 (a.k.a. CTNNB, EVR7, MRD19, NEDSDV, armadillo)
  • Promoter EF1a
  • Tag / Fusion Protein
    • Myc (N terminal on insert)

Cloning Information

Resource Information

Depositor Comments

The pLV-neo vector was modified from the attached vector by replacing dsRed with Neomycin resistant cassette and removing tTR-kRAB.

Addgene QC sequence analysis identified a S374G discrepancy, relative to reference sequence NP_001091679.1.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLV-beta-catenin deltaN90 was a gift from Bob Weinberg (Addgene plasmid # 36985 ; http://n2t.net/addgene:36985 ; RRID:Addgene_36985)
  • For your References section:

    Slug and Sox9 cooperatively determine the mammary stem cell state. Guo W, Keckesova Z, Donaher JL, Shibue T, Tischler V, Reinhardt F, Itzkovitz S, Noske A, Zurrer-Hardi U, Bell G, Tam WL, Mani SA, van Oudenaarden A, Weinberg RA. Cell. 2012 Mar 2;148(5):1015-28. 10.1016/j.cell.2012.02.008 PubMed 22385965