165,187 results

1. pBzCas13b-gRNA-NT

   Plasmid

   #164861

   Purpose

   Constitutive expression of single-spacer CRISPR array with non-targeting spacer for BzCas13b in bacteria.

   Depositor

   Chase Beisel

   Article

   Wandera et al Methods Mol Biol. 2022;2404:135-153. doi

   Insert

   BzCas13b-gRNA-NT
   

   Expression

   Bacterial

   Promoter

   J23119

   Available Since

   Feb. 22, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pBzCas13b-gRNA-1

   Plasmid

   #164858

   Purpose

   Constitutive expression of single-spacer CRISPR array with spacer #1 targeting deGFP mRNA for BzCas13b in bacteria.

   Depositor

   Chase Beisel

   Article

   Wandera et al Methods Mol Biol. 2022;2404:135-153. doi

   Insert

   BzCas13b-gRNA-1
   

   Expression

   Bacterial

   Promoter

   J23119

   Available Since

   Feb. 22, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. GFP-BRD9

   Plasmid

   #65380

   Purpose

   GFP-tagged BRD protein, which can be used to be expressed in mammalian cells.

   Depositor

   Kyle Miller

   Article

   Gong et al Genes Dev. 2015 Jan 15;29(2):197-211. do

   Insert

   BRD9 (BRD9 Human)
   

   Tags

   EmGFP and V5

   Expression

   Mammalian

   Mutation

   none

   Promoter

   CMV

   Available Since

   May 14, 2015

   Availability

   Academic Institutions and Nonprofits only

4. pDule-tfmF A65V S158A

   Plasmid

   #85484

   Purpose

   Machinery plasmid expressing permissive Mj tfmF synthetase and cognate amber suppressing tRNA

   Depositor

   Ryan Mehl

   Article

   Miyake-Stoner et al Biochemistry. 2010 Mar 2;49(8):1667-77.

   Insert

   tri-fluoromethyl-phenylalanine Mj synthetase A65V S158A
   

   Tags

   None

   Expression

   Bacterial

   Mutation

   Y32V F108W Q109M I159M with 65Val and 158Ala

   Promoter

   lpp (constitutive)

   Available Since

   Jan. 10, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. p156

   Plasmid

   #186179

   Purpose

   Shotgun Metagenomic Spike-in

   Depositor

   Karsten Zengler

   Article

   Zaramela et al mSystems. 2022 Dec 20;7(6):e0044722. doi

   Insert

   synDNA 156
   

   Use

   Synthetic Biology and Unspecified; Cloning

   Available Since

   Sept. 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. p136

   Plasmid

   #186177

   Purpose

   Shotgun Metagenomic Spike-in

   Depositor

   Karsten Zengler

   Article

   Zaramela et al mSystems. 2022 Dec 20;7(6):e0044722. doi

   Insert

   synDNA 136
   

   Use

   Synthetic Biology; Cloning

   Available Since

   Sept. 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA3.1(+)-PM-RasAR

   Plasmid

   #205569

   Purpose

   Plasma membrane-targeted RasAR.

   Depositor

   Jin Zhang

   Article

   Weeks et al J Am Chem Soc. 2022 Sep 28;144(38):17432

   Insert

   PM-RasAR (RAF1 Human, Synthetic)
   

   Tags

   N-terminal targeting sequence from Lyn kinase

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Oct. 2, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pINDUCER21-MYB

   Plasmid

   #51305

   Purpose

   Expression of MYB after doxycycline administration

   Depositor

   George Daley

   Article

   Doulatov et al Cell Stem Cell. 2013 Oct 3;13(4):459-70.

   Insert

   v-myb avian myeloblastosis viral oncogene homolog (MYB Human)
   

   Use

   Lentiviral

   Tags

   n/a

   Mutation

   full length ORF

   Available Since

   June 13, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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9. FlucSM-HA-GFP11-N1

   Plasmid

   #91955

   Purpose

   Expresses aggregation-prone FlucSM-HA-GFP11 in mammalian cells

   Depositor

   Rong Li

   Article

   Ruan et al Nature. 2017 Mar 16;543(7645):443-446. d

   Insert

   FlucSM-HA-GFP11
   

   Expression

   Mammalian

   Mutation

   R188Q

   Promoter

   CMV

   Available Since

   June 6, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pMT010

    Plasmid

    #64762

    Purpose

    Recombination-Induced Tag Exchange (RITE) Cassette, HA to a 2xFLAG epitope tag, Hygro selection

    Depositor

    Fred van Leeuwen

    Article

    Terweij et al G3 (Bethesda). 2013 Aug 7;3(8):1261-72.

    Insert

    RITE cassette
    

    Use

    Rite cassette

    Promoter

    none

    Available Since

    July 6, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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11. pHR-UCOE-SFFV-Zim3-dCas9-P2A-mCherry

    Plasmid

    #188779

    Purpose

    dCas9 with an N-term Zim3 KRAB-NLS fusion, a C-term HA-2xNLS

    Depositor

    Marco Jost, Jonathan Weissman

    Article

    Replogle et al eLife. 2022 Dec 28;11:e81856. doi: 10.75

    Insert

    Zim3-dCas9
    

    Use

    Lentiviral

    Tags

    Zim3 KRAB-NLS fusion

    Promoter

    SFFV

    Available Since

    Oct. 18, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pME-puro-SNAP-FLAG-CD59

    Plasmid

    #50374

    Purpose

    Express SNAP and FLAG-tagged human CD59, a GPI-AP in mammmalian cells

    Depositor

    Reika Watanabe

    Article

    Castillon et al Mol Biol Cell. 2013 Jun;24(12):2021-33.

    Insert

    SNAP
    

    Expression

    Mammalian

    Promoter

    SR alpha

    Available Since

    July 15, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCDH-puro-Bcl-XL

    Plasmid

    #46972

    Purpose

    lentiviral expression of Bcl-XL

    Depositor

    Jialiang Wang

    Article

    Cheng et al Clin Cancer Res. 2013 Apr 1;19(7):1748-5

    Insert

    BCL2L1 (BCL2L1 Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 7, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCalN-ddGFP-A

    Plasmid

    #40290

    Depositor

    Robert Campbell

    Article

    Alford et al ACS Synth Biol. 2012 Dec 21;1(12):569-75

    Insert

    Calnexin (CANX Human)
    

    Tags

    ddGFP-A

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 21, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcDNA3.1/Puro-CAG-JEDI-1P

    Plasmid

    #202607

    Purpose

    Genetically encoded voltage indicator (GEVI) JEDI-1P, expressed under strong mammalian promoter (CAG)

    Depositor

    Francois St-Pierre

    Article

    Lu et al Nat Commun. 2023 Oct 12;14(1):6423. doi:

    Insert

    JEDI-1P
    

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    June 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pHyg-AID*-6FLAG

    Plasmid

    #99519

    Purpose

    C-terminal AID*-6FLAG degron cassette with HygR marker for S. cerevisiae

    Depositor

    Helle Ulrich

    Article

    Morawska et al Yeast. 2013 Sep;30(9):341-51. doi: 10.10

    Insert

    IAA17(71-116) (AXR3 Mustard Weed)
    

    Use

    Pcr template for yeast tagging cassette

    Tags

    6xFLAG

    Available Since

    Sept. 14, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. human STIM1 CFP

    Plasmid

    #19755

    Depositor

    Anjana Rao

    Article

    Prakriya et al Nature. 2006 Sep 14. 443(7108):230-3.

    Insert

    STIM1 (STIM1 Human)
    

    Use

    Retroviral

    Tags

    CFP

    Expression

    Mammalian

    Available Since

    Oct. 27, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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18. PB_iMYOD1_P2A_GFP_Puro

    Plasmid

    #168804

    Purpose

    PiggyBac transposon construct with doxycycline inducible MYOD1 + GFP expression

    Depositor

    Prashant Mali

    Article

    Dailamy et al Stem Cell Reports. 2021 Sep 8. pii: S221

    Insert

    MYOD1 (MYOD1 Human, Synthetic)
    

    Use

    Synthetic Biology

    Expression

    Mammalian

    Promoter

    TRE

    Available Since

    Aug. 31, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCRISPR-HOT-mNeon-P2A-T2A-mCherry-CAAX

    Plasmid

    #240799

    Purpose

    CRISPR-HOT donor plasmid for mNeonGreen fusion protein co-expressing membrane-bound mCherry via a P2A-T2A tandem

    Depositor

    Hans Clevers

    Article

    Krueger et al Science. 2025 Sep 4;389(6764):eadr8753.

    Insert

    mNeonGreen-mCherry-CAAX
    

    Use

    CRISPR

    Expression

    Mammalian

    Mutation

    none

    Available Since

    Aug. 4, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCRISPR-HOT-mNeon-Stop-PGK-mCherry-CAAX

    Plasmid

    #240800

    Purpose

    CRISPR-HOT donor plasmid for mNeonGreen fusion protein co-expressing membrane-bound mCherry via PGK promoter

    Depositor

    Hans Clevers

    Article

    Krueger et al Science. 2025 Sep 4;389(6764):eadr8753.

    Insert

    mNeonGreen mCherry-CAAX
    

    Use

    CRISPR

    Expression

    Mammalian

    Mutation

    none

    Promoter

    PGK

    Available Since

    Aug. 1, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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