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179,343 results

Showing: 10501 - 10520 of 179343 results

  1. pcDNA C12 ecDHFR DD YFP HA

    Plasmid
    #192815
    Purpose
    Expresses an N-terminal enhanced destabilizing domain (DD) version of E. coli DHFR with higher basal turnover in mammalian systems. Contains missense mutations W74R/T113S/E120D/Q146L.
    Depositor
    John Hulleman
    Publication
    Nakahara et al ACS Chem Biol. 2022 Sep 19. doi: 10.1021
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    E. coli dihydrofolate reductase
    Tags
    enhanced yellow fluorescent protein and hemagglut…
    Expression
    Mammalian
    Mutation
    W74R/T113S/E120D/Q146L
    Promoter
    CMV
    Available since
    Nov. 14, 2022
    Availability
    Academic Institutions and Nonprofits only
    192815-plasmid-map-sequence-id-377240

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  5. EB3N-VVDfast-mCl3-iLID

    Plasmid
    #190165
    Purpose
    Expresses EB3N-VVDfast-mCl3-iLID, a microtubule anchor used for optogenetic recruitment of SspB-mCh-p60, in mammalian cells.
    Depositor
    Anna Akhmanova
    Publication
    Meiring et al Curr Biol. 2022 Nov 7;32(21):4660-4674.e
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    EB3N-VVDfast-mClover3-iLID (MAPRE3 Human, Synthetic)
    Tags
    -VVDfast-mClover3-iLID
    Expression
    Mammalian
    Mutation
    1-189 aa from EB3
    Available since
    Oct. 11, 2022
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    190165-plasmid-map-sequence-id-374287

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  9. pYPQ-Cas9-Act3.0

    Plasmid
    #178954
    Purpose
    It consists of a catalytically active Cas9 nuclease and MS2-SunTag-activators activation complex (ScFv-sfGFP-2xTAD), enabling simultaneous genome editing and gene activation.
    Depositor
    Yiping Qi
    Publication
    Pan et al Nat Plants. 2022 May;8(5):513-525. doi:
    Insert
    zCas9-T2A-MS2-SunTag-rbcS-E9t-ter-ZmUbi-scFv-sfGFP-2xTAD-GB1-NOS-ter
    Use
    CRISPR
    Expression
    Plant
    Available since
    May 23, 2022
    Availability
    Academic Institutions and Nonprofits only
    178954-plasmid-map-sequence-id-354300

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  10. pPB-Ins-TRE3Gp-dCas9-Suntag-VP64-EF1Ap-Puro

    Plasmid
    #183409
    Purpose
    piggyBAC-based doxycycline-inducible dCas9-5xSuntag-VP64 CRISPR activation plasmid for enhancer and promoter activation in mammalian cells
    Depositor
    Azim Surani
    Publication
    Tang et al Nat Cell Biol. 2022 Apr;24(4):448-460. d
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    dCas9-5xGCN5-P2A-scFV-sfGFP-VP64-GB1
    Use
    CRISPR and Synthetic Biology; Piggybac
    Expression
    Mammalian
    Promoter
    TRE3G
    Available since
    May 6, 2022
    Availability
    Academic Institutions and Nonprofits only
    183409-plasmid-map-sequence-id-361258

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  12. pHN781

    Plasmid
    #178828
    Purpose
    Bacterial expression for human Saposin A
    Depositor
    Kazuhiro Abe
    Insert
    artificial gene encoding human Saposin A (AR Human)
    Tags
    His8, TEV
    Expression
    Bacterial
    Mutation
    Codon-usage was optimized for bacterial expressio…
    Promoter
    T7
    Available since
    Jan. 12, 2022
    Availability
    Academic Institutions and Nonprofits only
    178828-plasmid-map-sequence-id-351929

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  14. VPS13D^EGFP

    Plasmid
    #174109
    Purpose
    The insert is a codon optimized DNA sequence encoding VPS13D with an internal EGFP.
    Depositor
    Pietro De Camilli
    Publication
    Guillen-Samander et al J Cell Biol. 2021 May 3;220(5). pii: 212
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Codon optimized VPS13D
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    With an internal EGFP tag in aminoacid position 1…
    Promoter
    CMV
    Available since
    Oct. 13, 2021
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    174109-plasmid-map-sequence-id-345250

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  17. pCAG R2pH-LAMP1-3xFLAG

    Plasmid
    #157940
    Purpose
    expresses ratiometric sensor of lysosomal lumenal pH
    Depositor
    Massimiliano Stagi
    Publication
    Ponsford et al Autophagy. 2020 Jun 9:1-19. doi: 10.1080
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    R2pH-LAMP1-3xFLAG (Lamp1 Mouse)
    Tags
    3XFLAG, mCherry, and pHluorin
    Expression
    Mammalian
    Promoter
    Chick beta actin pomoter
    Available since
    Sept. 24, 2020
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    157940-plasmid-map-sequence-id-315065

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  19. Sc++

    Plasmid
    #155011
    Purpose
    Expresses the codon-optimized, engineered Sc++ Cas9 nuclease in mammalian cells
    Depositor
    Joseph Jacobson
    Publication
    Chatterjee et al Nat Biotechnol. 2020 May 11. pii: 10.103
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Sc++
    Expression
    Mammalian
    Mutation
    T1227K, Loop Substitution from S. anginosus
    Promoter
    EF1ɑ
    Available since
    Sept. 11, 2020
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    155011-plasmid-map-sequence-id-308829

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  20. GFP-OMP25

    Plasmid
    #141150
    Purpose
    Fluorescent labeling of the outer mitochondrial membrane (OMM) in mammalian cells
    Depositor
    Gia Voeltz
    Publication
    Abrisch et al J Cell Biol. 2020 Apr 6;219(4). pii: 133
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    OMP25 (Synj2bp Rat)
    Tags
    AcGFP
    Expression
    Mammalian
    Mutation
    OMP25 c terminus from addgene plasmid #69598
    Promoter
    CMV
    Available since
    Aug. 31, 2020
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    141150-plasmid-map-sequence-id-309903

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Showing: 10501 - 10520 of 179343 results