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Addgene

pCFJ782 - HygroR
(Plasmid #190933)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 190933 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pDONR1-2
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 2544
  • Total vector size (bp) 4281
  • Vector type
    Worm Expression
  • Selectable markers
    Hygromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Hygromycin resistance gene
  • Alt name
    HygroR
  • Species
    C. elegans (nematode)
  • Promoter rps-0

Cloning Information

Resource Information

  • Supplemental Documents
  • A portion of this plasmid was derived from a plasmid made by
    The hygromycin resistance gene was originally developed by the Chin lab (Cambridge U) in the publication Radman et al. (2013) Plos One (https://pubmed.ncbi.nlm.nih.gov/24130756/).

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

The plasmid can be used as a co-injection marker to select for transgenic C. elegans using hygromycin B selection.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCFJ782 - HygroR was a gift from Christian Froekjaer-Jensen (Addgene plasmid # 190933 ; http://n2t.net/addgene:190933 ; RRID:Addgene_190933)
  • For your References section:

    Modular safe-harbor transgene insertion (MosTI) for targeted single-copy and extrachromosomal array integration in C. elegans. El Mouridi S, Alkhaldi F, Frokjaer-Jensen C. G3 (Bethesda). 2022 Jul 28. pii: 6651068. doi: 10.1093/g3journal/jkac184. 10.1093/g3journal/jkac184 PubMed 35900171