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Optimization of S. aureus dCas9 and CRISPRi Elements for a Single Adeno-Associated Virus that Targets an Endogenous Gene.
Backstrom JR, Sheng J, Wang MC, Bernardo-Colon A, Rex TS
Mol Ther Methods Clin Dev. 2020 Sep 6;19:139-148. doi: 10.1016/j.omtm.2020.09.001. eCollection 2020 Dec 11.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
163020(326) pAAV SV40-ZsGreen U6-gRNAFluorescent reporter for Sa gRNA (Bsa1 sites)
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163021(401) pAAV CMV-ZsGreen U6-gRNAFluorescent reporter for Sa gRNA (Bsa1 sites)
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163022(364) SB KRAB-SadCas9Sleeping Beauty TET-On expression of CRISPRi (without U6-gRNA)
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163023(517) SB KRAB-SadCas9 U6-gStopSleeping Beauty TET-On expression of CRISPRi with gSTOP gRNA
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163024(559) pAAV EF-1a KRAB-SadCas9 U6-gRNAExpression of CRISPRi with U6-gRNA (Bsa1 sites)
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163025(564) pAAV Alb-AAT KRAB-SadCas9 U6-mPcsk9Expression of CRISPRi with gRNA against mouse Pcsk9
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163030(565) pAAV Alb-AAT KRAB-SadCas9 U6-gSTOPExpression of CRISPRi with gSTOP gRNA
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