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(564) pAAV Alb-AAT KRAB-SadCas9 U6-mPcsk9
(Plasmid #163025)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 163025 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    AAV2
  • Backbone manufacturer
    Cell Biolabs
  • Backbone size w/o insert (bp) 3500
  • Total vector size (bp) 7286
  • Vector type
    Mammalian Expression, AAV

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Growth instructions
    34-35 degrees is ideal
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Mammalian codon-optimized SadCas9
  • Species
    S. aureus
  • Insert Size (bp)
    3681
  • Entrez Gene
    Pcsk9 (a.k.a. AI415265, AI747682, FH3, HCHOLA3, Na, Narc1, PC9)
  • Promoter Alb-AAT
  • Tags / Fusion Proteins
    • KRAB (N terminal on insert)
    • myc NLS (C terminal on insert)
    • SV40 pA (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoR1 (not destroyed)
  • 3′ cloning site Kpn1 (not destroyed)
  • 5′ sequencing primer 5-Cas-Seq-A
  • 3′ sequencing primer SVpa-Seq-A
  • (Common Sequencing Primers)

Resource Information

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    (564) pAAV Alb-AAT KRAB-SadCas9 U6-mPcsk9 was a gift from Tonia Rex (Addgene plasmid # 163025 ; http://n2t.net/addgene:163025 ; RRID:Addgene_163025)
  • For your References section:

    Optimization of S. aureus dCas9 and CRISPRi Elements for a Single Adeno-Associated Virus that Targets an Endogenous Gene. Backstrom JR, Sheng J, Wang MC, Bernardo-Colon A, Rex TS. Mol Ther Methods Clin Dev. 2020 Sep 6;19:139-148. doi: 10.1016/j.omtm.2020.09.001. eCollection 2020 Dec 11. 10.1016/j.omtm.2020.09.001 PubMed 33024795