Addgene: Development of a New DHFR-Based Destabilizing Domain with Enhanced Basal Turnover and Applicability in Mammalian Systems.

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John Hulleman
Nakahara et al

Development of a New DHFR-Based Destabilizing Domain with Enhanced Basal Turnover and Applicability in Mammalian Systems.
Nakahara E, Mullapudi V, Collier GE, Joachimiak LA, Hulleman JD
ACS Chem Biol. 2022 Sep 19. doi: 10.1021/acschembio.2c00518. PubMed Article

Plasmids from Article

ID	Plasmid	Purpose
192815	pcDNA C12 ecDHFR DD YFP HA	Expresses an N-terminal enhanced destabilizing domain (DD) version of E. coli DHFR with higher basal turnover in mammalian systems. Contains missense mutations W74R/T113S/E120D/Q146L.

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