|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||11547||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 11600
Growth in Bacteria
Gene/Insert nameProtein Kinase B
SpeciesM. musculus (mouse)
Insert Size (bp)1500
Entrez GeneAkt1 (a.k.a. Ak, Akt, LTR-akt, PK, PKB, PKB/A, PKB/Akt, PKBalpha, Rac)
/ Fusion Proteins
- myr signal (N terminal on insert)
- HA tag (N terminal on insert)
- Cloning method Restriction Enzyme
- 5′ cloning site Pme1 (destroyed during cloning)
- 3′ cloning site Pme1 (destroyed during cloning)
- 5′ sequencing primer RCAS-F (Common Sequencing Primers)
The RCAS vectors that carry oncogenes are harmless for mammalian cells, but can be oncogenic in avian cells since some avian
cells express the receptor for the RCAS virus.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:RCAS-myrAkt was a gift from Sandra Orsulic (Addgene plasmid # 11547 ; http://n2t.net/addgene:11547 ; RRID:Addgene_11547)
For your References section:Induction of ovarian cancer by defined multiple genetic changes in a mouse model system. Orsulic S, Li Y, Soslow RA, Vitale-Cross LA, Gutkind JS, Varmus HE. Cancer Cell. 2002 Feb . 1(1):53-62. 10.1016/S1535-6108(01)00002-2 PubMed 12086888