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Addgene

pWC2
(Plasmid #15960)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 15960 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pT7-7
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Kinesin 1-401 BCCP his tag
  • Alt name
    K401-BIO-H6
  • Alt name
    kinesin heavy chain
  • Species
    D. melanogaster (fly); E. coli
  • Mutation
    amino acids 1-401
  • GenBank ID
    M24441
  • Entrez Gene
    Khc (a.k.a. Dmel_CG7765, 2R6, CG7765, DK, DKH, Dm KHC, DmK, DmKHC, Dmel\CG7765, Dmkin, KHC, KIF 5A, KIF5, KIF5B, KIN, Kif5, Kin, Kin-1, Kinesin-1, khc, kin, kinesin, kinesin-1, l(2)W12, l(2)k13219, l(2)k13314, l(2R)W12, pgs)
  • Tags / Fusion Proteins
    • BCCP (C terminal on insert)
    • His6 (C terminal on insert)

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pWC2 was a gift from Jeff Gelles (Addgene plasmid # 15960 ; http://n2t.net/addgene:15960 ; RRID:Addgene_15960)
  • For your References section:

    Two Distinct Modes of Processive Kinesin Movement in Mixtures of ATP and AMP-PNP. Subramanian R, Gelles J. J Gen Physiol. 2007 Nov . 130(5):445-55. 10.1085/jgp.200709866 PubMed 17968024