pSCW02
(Plasmid #72301)

• Purpose: (Empty Backbone) Starting substrate for in vitro DNA mismatch repair substrate monitored by FauI cleavage. Derived from pUC19CPDrev H. HWang and J.B. Hays 2002 JBC 277:26136.
• Depositing Lab: Peggy Hsieh
• Publication: Geng et al Anal Biochem. 2011 Jun 15;413(2):179-84. doi: 10.1016/j.ab.2011.02.017. Epub 2011 Feb 15.

Backbone

• Vector backbone: pUC19CPDrev
• Backbone manufacturer: gift from Dr. John Hays
• Backbone size (bp): 2025
• Modifications to backbone: pSCW02 were derived from pUC19CPDrev and follow the original numbering system with the four Nt.BstNBI endonuclease sites residing on the antisense strand as defined by the pUC19 bla gene in pSCW02 (Fig. 1A). A BbvC1 restriction endonuclease site was introduced at position 55 (Stratagene QuikchangeXL) in pUC19CPDrev and an existing BbvC1 site removed between positions 331 and 348. The resulting plasmid was digested with AatII and SapI, gel purified to remove the 76-bp intervening sequence, and ligated to synthetic 76-bp duplexes to create a 2030-bp plasmid, pSCW02, that served as homoduplex DNA controls in MMR assays. The plasmid was used to create circular DNA mismatch substrates for mismatch repair protein research
• Vector type: Bacterial Expression ; derivative of pUC19
• Promoter: lac

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry