Full plasmid sequence is not available for this item.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||12453||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 4679
Vector typeMammalian Expression
Growth in Bacteria
Copy numberHigh Copy
Gene/Insert name14-3-3 sigma
SpeciesH. sapiens (human)
Entrez GeneSFN (a.k.a. YWHAS)
/ Fusion Protein
- FLAG (N terminal on backbone)
- Cloning method Restriction Enzyme
- 5′ cloning site HindIII (not destroyed)
- 3′ cloning site XbaI (not destroyed)
- 5′ sequencing primer pCEP fwd (Common Sequencing Primers)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pFlagCMV2-14-3-3sigma was a gift from Dong-Er Zhang (Addgene plasmid # 12453 ; http://n2t.net/addgene:12453 ; RRID:Addgene_12453)
For your References section:The interferon-inducible ubiquitin-protein isopeptide ligase (E3) EFP also functions as an ISG15 E3 ligase. Zou W, Zhang DE. J Biol Chem. 2006 Feb 17. 281(7):3989-94. 10.1074/jbc.M510787200 PubMed 16352599