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(Plasmid #22884)


Item Catalog # Description Quantity Price (USD)
Plasmid 22884 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.


  • Vector backbone
    pBluescript SK (-)
  • Backbone manufacturer
  • Backbone size w/o insert (bp) 9000
  • Vector type
    Cre/Lox ; homologous recombination

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
  • Growth Strain(s)
  • Copy number
    Low Copy


  • Gene/Insert name
    hypoxanthine phosphoribosyltransferase-1
  • Alt name
  • Species
    H. sapiens (human)
  • Insert Size (bp)
  • Mutation
    PGK, NeoR, and BGH polyA are between the two genomic sequences (one section is 9.1kb and one is 2.5kb).
  • GenBank ID
  • Entrez Gene
    HPRT1 (a.k.a. HGPRT, HPRT)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site SalI and XmaI (not destroyed)
  • 3′ cloning site EcoRI and NotI (not destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer pLac
  • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Note that very little of the insert has been sequenced by Addgene. Please be sure to do your own sequencing of HPRT before using this plasmid.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    HPRT was a gift from James Thomson (Addgene plasmid # 22884 ; ; RRID:Addgene_22884)
  • For your References section:

    Homologous recombination in human embryonic stem cells. Zwaka TP, Thomson JA. Nat Biotechnol. 2003 Mar . 21(3):319-21. 10.1038/nbt788 PubMed 12577066