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pUNIV backbone
(Plasmid #24705)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 24705 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pCI-neo
  • Backbone manufacturer
    Invitrogen
  • Backbone size (bp) 5653
  • Vector type
    Mammalian Expression, Bacterial Expression, Yeast Expression ; Xenopus oocyte expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    XL1 Blue
  • Growth instructions
    Age I XL1-Blue
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    None
  • GenBank ID
    EF177812

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site MluI (not destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer TATGTAGCTTAGAGACTCCATTCGGGTGTTC
  • (Common Sequencing Primers)

Resource Information

  • Terms and Licenses
  • Industry Terms
    • Not Available to Industry
  • Articles Citing this Plasmid
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pUNIV backbone was a gift from Cynthia Czajkowski (Addgene plasmid # 24705 ; http://n2t.net/addgene:24705 ; RRID:Addgene_24705)
  • For your References section:

    Optimized expression vector for ion channel studies in Xenopus oocytes and mammalian cells using alfalfa mosaic virus. Venkatachalan SP, Bushman JD, Mercado JL, Sancar F, Christopherson KR, Boileau AJ. Pflugers Arch. 2007 Apr . 454(1):155-63. 10.1007/s00424-006-0183-1 PubMed 17146677