Full plasmid sequence is not available for this item.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||15034||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 3000
Vector typeBacterial Expression
Growth in Bacteria
Copy numberHigh Copy
Gene/Insert namebeta-globin 5' and 3' UTR
SpeciesX. laevis (frog)
Insert Size (bp)260
- Cloning method Restriction Enzyme
- 5′ cloning site HindIII (not destroyed)
- 3′ cloning site XbaI (not destroyed)
- 5′ sequencing primer SP6 (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
Vector with Xenopus 5' and 3' untranslated beta-globin sequences to enhance translation of synthetic mRNAs. Derivative of pSP64T that includes BglII, EcoRV, and SpeI insertion sites and stop codons for all three reading frames downstream of the cloning sites. See author's map for more information.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pSP64TS (DM#117) was a gift from Douglas Melton (Addgene plasmid # 15034 ; http://n2t.net/addgene:15034 ; RRID:Addgene_15034)
For your References section:Functional messenger RNAs are produced by SP6 in vitro transcription of cloned cDNAs. Krieg PA, Melton DA. Nucleic Acids Res. 1984 Sep 25. 12(18):7057-70. 10.1093/nar/12.18.7057 PubMed 6207484
Map uploaded by the depositor.