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Addgene

pSGEMKv10.1
(Plasmid #85704)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 85704 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pSGEM
  • Vector type
    Xenopus expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    KCNH1
  • Alt name
    Eag1
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    3000
  • Entrez Gene
    KCNH1 (a.k.a. EAG, EAG1, Kv10.1, TMBTS, ZLS1, h-eag, hEAG, hEAG1)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site unknown (unknown if destroyed)
  • 3′ cloning site unknown (unknown if destroyed)
  • 5′ sequencing primer M13-F20
  • 3′ sequencing primer pBluescript-KS
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

pSGEM vector, 3118 bp, engineered by Michael Hollmann & Dorothea Gremmels, March 1994. pSGEM is based on the pGEMHE-MCS vector (3096 bp) engineered by M.Hollmann. pGEMHE-MCS itself is based on the pGEMHE vector (3022 bp) which in turn is derived from pGEM-3Z of Promega (2743 bp) See also Liman,E.R., Tytgat,J. and Hess,P. Neuron (1992) Vol.9, pp.861-871

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pSGEMKv10.1 was a gift from Luis Pardo (Addgene plasmid # 85704 ; http://n2t.net/addgene:85704 ; RRID:Addgene_85704)
  • For your References section:

    C-terminal domains implicated in the functional surface expression of potassium channels. Jenke M, Sanchez A, Monje F, Stuhmer W, Weseloh RM, Pardo LA. EMBO J. 2003 Feb 3;22(3):395-403. 10.1093/emboj/cdg035 PubMed 12554641